CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017455
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mono [ADP-ribose] polymerase PARP16 
Protein Synonyms/Alias
 ADP-ribosyltransferase diphtheria toxin-like 15; ARTD15; Poly [ADP-ribose] polymerase 16; PARP-16 
Gene Name
 PARP16 
Gene Synonyms/Alias
 C15orf30 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35ASALQSYKRDSVLRPubiquitination[1, 2]
142NAKFYETKGERDLIYubiquitination[3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Mono-ADP-ribosyltransferase targeting the karyopherin KPNB1. Plays a role in unfolded protein response (UPR), by ADP- ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors. 
Sequence Annotation
 DOMAIN 5 91 PARP alpha-helical.
 DOMAIN 94 279 PARP catalytic.
 BINDING 152 152 NAD.
 BINDING 182 182 NAD.
 BINDING 254 254 NAD; may stabilize a reaction  
Keyword
 3D-structure; ADP-ribosylation; Alternative splicing; Complete proteome; Endoplasmic reticulum; Glycosyltransferase; Membrane; NAD; Nucleus; Polymorphism; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 322 AA 
Protein Sequence
MQPSGWAAAR EAAGRDMLAA DLRCSLFASA LQSYKRDSVL RPFPASYARG DCKDFEALLA 60
DASKLPNLKE LLQSSGDNHK RAWDLVSWIL SSKVLTIHSA GKAEFEKIQK LTGAPHTPVP 120
APDFLFEIEY FDPANAKFYE TKGERDLIYA FHGSRLENFH SIIHNGLHCH LNKTSLFGEG 180
TYLTSDLSLA LIYSPHGHGW QHSLLGPILS CVAVCEVIDH PDVKCQTKKK DSKEIDRRRA 240
RIKHSEGGDI PPKYFVVTNN QLLRVKYLLV YSQKPPKRAS SQLSWFSSHW FTVMISLYLL 300
LLLIVSVINS SAFQHFWNRA KR 322 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0003956; F:NAD(P)+-protein-arginine ADP-ribosyltransferase activity; IEA:EC.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IEA:InterPro.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR012317; Poly(ADP-ribose)pol_cat_dom. 
Pfam
 PF00644; PARP 
SMART
  
PROSITE
 PS51060; PARP_ALPHA_HD
 PS51059; PARP_CATALYTIC 
PRINTS