CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033357
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 COP9 signalosome complex subunit 1 
Protein Synonyms/Alias
 G protein pathway suppressor 1, isoform CRA_b 
Gene Name
 GPS1 
Gene Synonyms/Alias
 hCG_1987516 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
121TRKKALLKLEKLDTDubiquitination[1]
124KALLKLEKLDTDLKNacetylation[2]
124KALLKLEKLDTDLKNubiquitination[3]
130EKLDTDLKNYKGNSIubiquitination[1, 3, 4, 5, 6]
133DTDLKNYKGNSIKESubiquitination[1, 3]
138NYKGNSIKESIRRGHubiquitination[1, 6]
163GDLSNALKCYSRARDubiquitination[1, 4, 7]
204HVLSYVSKAESTPEIubiquitination[1, 3, 5, 6, 7, 8]
228QTQAILTKLKCAAGLacetylation[2]
228QTQAILTKLKCAAGLubiquitination[1, 3, 4, 5, 7, 9]
230QAILTKLKCAAGLAEubiquitination[1, 6]
317IFKFYESKYASCLKMubiquitination[1]
402ARVDSHSKILYARDVubiquitination[1, 3, 4, 5, 6, 7, 8]
418QRSTTFEKSLLMGKEubiquitination[1, 3, 4, 5, 6]
424EKSLLMGKEFQRRAKubiquitination[1, 3, 4, 6]
431KEFQRRAKAMMLRAAubiquitination[1]
447LRNQIHVKSPPREGSubiquitination[1, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MQIDVDPQED PQNAPDVNYV VENPSLDLEQ YAASYSGLMR IERLQFIADH CPTLRVEALK 60
MALSFVQRTF NVDMYEEIHR KLSEATRELQ NAPDAIPESG VEPPALDTAW VEATRKKALL 120
KLEKLDTDLK NYKGNSIKES IRRGHDDLGD HYLDCGDLSN ALKCYSRARD YCTSAKHVIN 180
MCLNVIKVSV YLQNWSHVLS YVSKAESTPE IAEQRGERDS QTQAILTKLK CAAGLAELAA 240
RKYKQAAKCL LLASFDHCDF PELLSPSNVA IYGGLCALAT FDRQELQRNV ISSSSFKLFL 300
ELEPQVRDII FKFYESKYAS CLKMLDEMKD NLLLDMYLAP HVRTLYTQIR NRALIQYFSP 360
YVSADMHRMA AAFNTTVAAL EDELTQLILE GLISARVDSH SKILYARDVD QRSTTFEKSL 420
LMGKEFQRRA KAMMLRAAVL RNQIHVKSPP REGSQGELTP ANSQSRMSTN M 471 
Gene Ontology
 GO:0008180; C:signalosome; IEA:Compara. 
Interpro
 IPR019585; 26S_proteasome_reg_su-Rpn7.
 IPR000717; PCI_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF01399; PCI
 PF10602; RPN7 
SMART
 SM00088; PINT 
PROSITE
  
PRINTS