CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006047
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit gamma 
Protein Synonyms/Alias
 Gamma-coat protein; Gamma-COP 
Gene Name
 SEC21 
Gene Synonyms/Alias
 YNL287W; N0543 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
8MSAHTYKKFENSTSGacetylation[1]
118QNGSDLIKPDAIRSLubiquitination[2]
243SMKNQLAKVELVKIVacetylation[1]
622KAINLKRKQEQIFNQubiquitination[2]
647EAESVPEKRADANSFubiquitination[2, 3]
910IKMIKSSKGLALKVHubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. 
Sequence Annotation
 REPEAT 258 296 HEAT 1.
 REPEAT 337 372 HEAT 2.
 REPEAT 373 410 HEAT 3.
 REPEAT 412 449 HEAT 4.
 REPEAT 524 562 HEAT 5.
 MOD_RES 638 638 Phosphothreonine.
 MOD_RES 643 643 Phosphoserine.
 MOD_RES 653 653 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; Endosome; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 935 AA 
Protein Sequence
MSAHTYKKFE NSTSGDLPDK MTIYQDCMNT FNESPVNSKR CRLLISRLLR LLAQGETFPQ 60
NEATALFFSI SKLFQHQNDP LRQAVYLAIK ELSGISEDVL MATSSIMKDV QNGSDLIKPD 120
AIRSLTYVLD ESTAFSAERL LKSAVVSRHP SISSAALCTS YHLLPISEVT IRRFTNETQE 180
AVLDLKQFPN QHGNSEYYPN STYISQYHAL GLLYQLKKTD KMALLKLVRH FSENNSMKNQ 240
LAKVELVKIV NDLIYRDPQL FSQFRPLLSD WLSNKFESVQ LETAKLITSF ATRNSRLVAP 300
ELYAAAISAL QSLLTVPRVS SRFAALRILN RISMVSPEKI VVCNPELESL INDSNRNIST 360
YAITTLLKTG TSKNISSLIS TITNFIHDVS DDFKIIIIDA VRTLSLNFPQ EWKSILNFLI 420
DVLKNSEGGF KFKNSIVEAL IDIVSFVPQS KELALENLCD FIEDCEFNEI LVRILHLLGK 480
EGPSAPNPSL YVRHIYNRVV LENSIIRSAA VVALSKFALT KNDPTLYESI ISLLKRIAND 540
KDDEVRDRAT IALEFIDSAR NKDDVIAQNL IESKYFYDIP SLESKLSSYI SSNTDSFATA 600
FDVNQVRKFT EDEMKAINLK RKQEQIFNQK SETTLDTTPE AESVPEKRAD ANSFAGPNLD 660
DHQEDLLATK YADELLSIEQ IKPFGQLVNS SRAISLTEPE AEFVVRGVKH LFKDNVVLQF 720
NITNTLTDIA LDNVSVVCTP EISDEAELEE LFTLQVDRLL PSEEAACYVA FKKLDEIVME 780
GFLNNLTFTT KEINPDTNEP FDGDEGFQDE YEIDSIFLNA GDYVKSSFTG NFSATFDELP 840
CEEVAVFNIQ EDLSIQEVVD KIILNSSCLP VESTQFAPSD SNSHTLKLFG KSALTGSKVA 900
LQIKMIKSSK GLALKVHGKG EDSLLCSDLV NGLMQ 935 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IDA:SGD.
 GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR017106; Coatomer_gsu.
 IPR014863; Coatomer_gsu_app.
 IPR013040; Coatomer_gsu_app_Ig-like-sub. 
Pfam
 PF01602; Adaptin_N
 PF08752; Gamma-COP 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS