CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017571
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NHL repeat-containing protein 2 
Protein Synonyms/Alias
  
Gene Name
 NHLRC2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
133EKVLDNIKSAVLRYNubiquitination[1, 2, 3, 4]
215KIGIKLYKDSLPPSPubiquitination[3]
303TENHLIRKIDLEAEKubiquitination[3]
329TDKEGGAKGEQQPISubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 43 200 Thioredoxin.
 REPEAT 212 254 NHL 1.
 REPEAT 265 307 NHL 2.
 REPEAT 335 369 NHL 3.
 REPEAT 409 439 NHL 4.
 REPEAT 461 505 NHL 5.
 REPEAT 518 562 NHL 6.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 726 AA 
Protein Sequence
MAAPGGRGRS LSGLLPAQTS LEYALLDAVT QQEKDSLVYQ YLQKVDGWEQ DLSVPEFPEG 60
LEWLNTEEPI SVYKDLCGKI VVLDFFTYCC INCIHLLPDL HALEHTYSDK DGLLIIGVHS 120
AKFPNEKVLD NIKSAVLRYN ITHPMVNDAD ASLWQELEVS CWPTLVILGP RGNMLFSLIG 180
EGHKDKLFLY TSIALKYYKD RGQIRDNKIG IKLYKDSLPP SPLLFPGKVT VDQVTDRLVI 240
ADTGHHRILV VWKNGQIQYS IGGPNPGRKD GIFSESTFNS PQGVAIMNNI IYVADTENHL 300
IRKIDLEAEK VSTVAGIGIQ GTDKEGGAKG EQQPISSPWD VVFGTSGSEV QRGDILWIAM 360
AGTHQIWALL LDSGKLPKKN ELTKGTCLRF AGSGNEENRN NAYPHKAGFA QPSGLSLASE 420
DPWSCLFVAD SESSTVRTVS LKDGAVKHLV GGERDPMNLF AFGDVDGVGI NAKLQHPLGV 480
TWDKKRNLLY VADSYNHKIK VVDPKTKNCT TLAGTGDTNN VTSSSFTEST FNEPGGLCIG 540
ENGELLYVAD TNNHQIKVMD LETKMVSVLP IFRSENAVVD GPFLVEKQKT LPKLPKSAPS 600
IRLSPVTACA GQTLQFKLRL DLPSGSKLTE GVSSCWFLTA EGNEWLLQGQ IAAGDIENIS 660
SQPTISLQIP DDCLSLEAIV SVSVFLYYCS ADSSACMMKA ILFSQPLQIT DTQQGCIAPV 720
ELRYVF 726 
Gene Ontology
  
Interpro
 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR001258; NHL_repeat.
 IPR013017; NHL_repeat_subgr.
 IPR012336; Thioredoxin-like_fold. 
Pfam
 PF01436; NHL 
SMART
  
PROSITE
 PS51125; NHL
 PS51352; THIOREDOXIN_2 
PRINTS