CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011012
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 A-kinase anchor protein 12 
Protein Synonyms/Alias
 AKAP-12; A-kinase anchor protein 250 kDa; AKAP 250; Gravin; Myasthenia gravis autoantigen 
Gene Name
 AKAP12 
Gene Synonyms/Alias
 AKAP250 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75QANDIGFKKVFKFVGubiquitination[1]
79IGFKKVFKFVGFKFTubiquitination[1]
84VFKFVGFKFTVKKDKubiquitination[1]
91KFTVKKDKTEKPDTVubiquitination[1]
199SETGSTFKKFFTQGWubiquitination[1]
219KTSFRKPKEDEVEASubiquitination[1]
278RLSAEYEKVELPSEEubiquitination[1]
297SQGPSEEKPAPLATEubiquitination[1]
334EERTEEQKTEVEETAubiquitination[1]
363PQEAEPAKELVKLKEubiquitination[1]
393PDEKVLSKPPEGVVSubiquitination[1]
574KPEEPKRKVDTSVSWubiquitination[1]
591LICVGSSKKRARRGSubiquitination[1]
665VSTWESFKRLVTPRKubiquitination[1]
702TPDTEPGKEESWVSIubiquitination[1]
710EESWVSIKKFIPGRRubiquitination[1]
769MEAQQAQKSAEQPEQubiquitination[1]
777SAEQPEQKAATEVSKubiquitination[1]
951VLQAVAEKVKEESQLubiquitination[1]
953QAVAEKVKEESQLPGubiquitination[1]
989QAEASGLKKETDVVLubiquitination[1]
1004KVDAQEAKTEPFTQGubiquitination[1]
1012TEPFTQGKVVGQTTPubiquitination[1]
1176ADQYADEKTKDVPFFubiquitination[1]
1450ELETKSSKLVQNIIQubiquitination[1]
1602VEPKEDEKGDDVDDPubiquitination[1]
1627DASGGLTKESPDTNGubiquitination[1]
1678KQERESAKSELTES*ubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). 
Sequence Annotation
 DOMAIN 604 634 AKAP 1.
 DOMAIN 753 783 AKAP 2.
 DOMAIN 798 828 AKAP 3.
 REGION 266 557 Involved in PKC-binding (Probable).
 REGION 1541 1554 RII-binding (Probable).
 MOD_RES 19 19 Phosphoserine (By similarity).
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 219 219 Phosphoserine.
 MOD_RES 371 371 Phosphoserine.
 MOD_RES 374 374 Phosphotyrosine (By similarity).
 MOD_RES 381 381 Phosphoserine.
 MOD_RES 483 483 Phosphoserine.
 MOD_RES 505 505 Phosphoserine.
 MOD_RES 557 557 Phosphoserine.
 MOD_RES 598 598 Phosphoserine.
 MOD_RES 612 612 Phosphoserine.
 MOD_RES 627 627 Phosphoserine.
 MOD_RES 629 629 Phosphoserine (By similarity).
 MOD_RES 645 645 Phosphoserine.
 MOD_RES 646 646 Phosphothreonine (By similarity).
 MOD_RES 648 648 Phosphoserine (By similarity).
 MOD_RES 696 696 Phosphoserine (By similarity).
 MOD_RES 697 697 Phosphoserine (By similarity).
 MOD_RES 698 698 Phosphoserine (By similarity).
 MOD_RES 1331 1331 Phosphoserine.
 MOD_RES 1395 1395 Phosphoserine.
 MOD_RES 1587 1587 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Myristate; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1782 AA 
Protein Sequence
MLGTITITVG QRDSEDVSKR DSDKEMATKS AVVHDITDDG QEETPEIIEQ IPSSESNLEE 60
LTQPTESQAN DIGFKKVFKF VGFKFTVKKD KTEKPDTVQL LTVKKDEGEG AAGAGDHKDP 120
SLGAGEAASK ESEPKQSTEK PEETLKREQS HAEISPPAES GQAVEECKEE GEEKQEKEPS 180
KSAESPTSPV TSETGSTFKK FFTQGWAGWR KKTSFRKPKE DEVEASEKKK EQEPEKVDTE 240
EDGKAEVASE KLTASEQAHP QEPAESAHEP RLSAEYEKVE LPSEEQVSGS QGPSEEKPAP 300
LATEVFDEKI EVHQEEVVAE VHVSTVEERT EEQKTEVEET AGSVPAEELV EMDAEPQEAE 360
PAKELVKLKE TCVSGEDPTQ GADLSPDEKV LSKPPEGVVS EVEMLSSQER MKVQGSPLKK 420
LFTSTGLKKL SGKKQKGKRG GGDEESGEHT QVPADSPDSQ EEQKGESSAS SPEEPEEITC 480
LEKGLAEVQQ DGEAEEGATS DGEKKREGVT PWASFKKMVT PKKRVRRPSE SDKEDELDKV 540
KSATLSSTES TASEMQEEMK GSVEEPKPEE PKRKVDTSVS WEALICVGSS KKRARRGSSS 600
DEEGGPKAMG GDHQKADEAG KDKETGTDGI LAGSQEHDPG QGSSSPEQAG SPTEGEGVST 660
WESFKRLVTP RKKSKSKLEE KSEDSIAGSG VEHSTPDTEP GKEESWVSIK KFIPGRRKKR 720
PDGKQEQAPV EDAGPTGANE DDSDVPAVVP LSEYDAVERE KMEAQQAQKS AEQPEQKAAT 780
EVSKELSESQ VHMMAAAVAD GTRAATIIEE RSPSWISASV TEPLEQVEAE AALLTEEVLE 840
REVIAEEEPP TVTEPLPENR EARGDTVVSE AELTPEAVTA AETAGPLGAE EGTEASAAEE 900
TTEMVSAVSQ LTDSPDTTEE ATPVQEVEGG VPDIEEQERR TQEVLQAVAE KVKEESQLPG 960
TGGPEDVLQP VQRAEAERPE EQAEASGLKK ETDVVLKVDA QEAKTEPFTQ GKVVGQTTPE 1020
SFEKAPQVTE SIESSELVTT CQAETLAGVK SQEMVMEQAI PPDSVETPTD SETDGSTPVA 1080
DFDAPGTTQK DEIVEIHEEN EVASGTQSGG TEAEAVPAQK ERPPAPSSFV FQEETKEQSK 1140
MEDTLEHTDK EVSVETVSIL SKTEGTQEAD QYADEKTKDV PFFEGLEGSI DTGITVSREK 1200
VTEVALKGEG TEEAECKKDD ALELQSHAKS PPSPVEREMV VQVEREKTEA EPTHVNEEKL 1260
EHETAVTVSE EVSKQLLQTV NVPIIDGAKE VSSLEGSPPP CLGQEEAVCT KIQVQSSEAS 1320
FTLTAAAEEE KVLGETANIL ETGETLEPAG AHLVLEEKSS EKNEDFAAHP GEDAVPTGPD 1380
CQAKSTPVIV SATTKKGLSS DLEGEKTTSL KWKSDEVDEQ VACQEVKVSV AIEDLEPENG 1440
ILELETKSSK LVQNIIQTAV DQFVRTEETA TEMLTSELQT QAHVIKADSQ DAGQETEKEG 1500
EEPQASAQDE TPITSAKEES ESTAVGQAHS DISKDMSEAS EKTMTVEVEG STVNDQQLEE 1560
VVLPSEEEGG GAGTKSVPED DGHALLAERI EKSLVEPKED EKGDDVDDPE NQNSALADTD 1620
ASGGLTKESP DTNGPKQKEK EDAQEVELQE GKVHSESDKA ITPQAQEELQ KQERESAKSE 1680
LTES 1684 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0051018; F:protein kinase A binding; TAS:ProtInc.
 GO:0007186; P:G-protein coupled receptor signaling pathway; TAS:ProtInc.
 GO:0030819; P:positive regulation of cAMP biosynthetic process; IMP:UniProtKB.
 GO:0010739; P:positive regulation of protein kinase A signaling cascade; IMP:UniProtKB.
 GO:0006605; P:protein targeting; IEA:InterPro. 
Interpro
 IPR001573; Pkinase-A_anch_WSK-motif.
 IPR018459; RII_binding_1. 
Pfam
 PF10522; RII_binding_1
 PF03832; WSK 
SMART
  
PROSITE
  
PRINTS