CPLM-018342

Genbank Nucleotide ID

Negative elongation factor B

Protein Synonyms/Alias

NELF-B; Cofactor of BRCA1

Homo sapiens (Human)

Position	Peptide	Type	References
85	SAIASEGKAEERYKK	ubiquitination	[1, 2, 3]
99	KLEDLLEKSFSLVKM	ubiquitination	[4, 5]
135	LKLVMADKELYRACA	ubiquitination	[2, 4, 5]
146	RACAVEVKRQIWQDN	ubiquitination	[2]
166	DEVSPLLKQYILEKE	ubiquitination	[1]
291	QGFLDGVKKGQEQVL	ubiquitination	[1]
361	MIDSQVFKEPKMEVE	ubiquitination	[1]
398	QKLPAEEKAPVSYPN	ubiquitination	[1, 6]
413	TLPESFTKFLQEQRM	ubiquitination	[1, 6, 7]
519	HPRVAPSKLEALQKA	acetylation	[3, 8, 9, 10]
519	HPRVAPSKLEALQKA	ubiquitination	[1, 2, 3]
525	SKLEALQKALEPTGQ	ubiquitination	[1]
538	GQSGEAVKELYSQLG	ubiquitination	[1]
555	LEQLDHRKPSPAQAA	ubiquitination	[6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[9] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

MOD_RES 519 519 N6-acetyllysine.
MOD_RES 557 557 Phosphoserine.

Acetylation; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:LIFEdb.
GO:0032021; C:NELF complex; IDA:UniProtKB.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:InterPro.
GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
GO:0022415; P:viral reproductive process; TAS:Reactome.