UniProt Accession

 ACOT4_MOUSE; Q8BWN8; Q8BJQ1; Q8BL20; Q9QYR8 

Genbank Protein ID

 AF180801; AF180799; AF180800; AK046630; AK050420; AK080883 

Genbank Nucleotide ID

 AAF13872.1; AAF13872.1; AAF13872.1; BAC32814.1; BAC34246.1; BAC38060.1 

Protein Name

 Acyl-coenzyme A thioesterase 4 

Protein Synonyms/Alias

 Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib 

Gene Name

 Acot4 

Gene Synonyms/Alias

 Pte1b; Pte2b 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
266	FSGNRHIKYKQTMIP	acetylation	[1]
268	GNRHIKYKQTMIPPL	acetylation	[2]
268	GNRHIKYKQTMIPPL	succinylation	[2]
313	PSMIPIEKAKGPILF	acetylation	[2, 3]
313	PSMIPIEKAKGPILF	succinylation	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (By similarity). 

Sequence Annotation

 MOTIF 419 421 Microbody targeting signal (Potential).
 ACT_SITE 232 232 Charge relay system (By similarity).
 ACT_SITE 326 326 Charge relay system (By similarity).
 ACT_SITE 360 360 Charge relay system (By similarity).  

Keyword

 Complete proteome; Hydrolase; Peroxisome; Reference proteome; Serine esterase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 421 AA 

Protein Sequence

Gene Ontology

 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0047617; F:acyl-CoA hydrolase activity; IDA:HGNC.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IEA:EC.
 GO:0004778; F:succinyl-CoA hydrolase activity; IEA:Compara.
 GO:0043649; P:dicarboxylic acid catabolic process; IEA:Compara.
 GO:0043648; P:dicarboxylic acid metabolic process; IDA:HGNC.
 GO:0001676; P:long-chain fatty acid metabolic process; IEA:Compara.
 GO:0032788; P:saturated monocarboxylic acid metabolic process; IEA:Compara.
 GO:0046459; P:short-chain fatty acid metabolic process; IDA:HGNC.
 GO:0006104; P:succinyl-CoA metabolic process; IDA:HGNC.
 GO:0032789; P:unsaturated monocarboxylic acid metabolic process; IEA:Compara.
 GO:0000038; P:very long-chain fatty acid metabolic process; IEA:Compara. 

Interpro

 IPR016662; Acyl-CoA_thioEstase_long-chain.
 IPR014940; BAAT_C.
 IPR006862; Thio_Ohase/aa_AcTrfase. 

Pfam

 PF08840; BAAT_C
 PF04775; Bile_Hydr_Trans 

SMART

  

PROSITE

  

PRINTS