CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010300
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 L-rhamnonate dehydratase 
Protein Synonyms/Alias
 RhamD 
Gene Name
 rhmD 
Gene Synonyms/Alias
 yfaW; b2247; JW2241 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
154VVGLPVYKLLGGAVRacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR). Can also dehydrate L-lyxonate, L- mannonate and D-gulonate, although less efficiently, but not 2- keto-4-hydroxyheptane-1,7-dioate. 
Sequence Annotation
 ACT_SITE 325 325 Proton acceptor.
 METAL 222 222 Magnesium (By similarity).
 METAL 248 248 Magnesium (By similarity).
 METAL 276 276 Magnesium (By similarity).
 BINDING 29 29 Substrate.
 BINDING 55 55 Substrate (By similarity).
 BINDING 345 345 Substrate (By similarity).  
Keyword
 3D-structure; Complete proteome; Lyase; Magnesium; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 401 AA 
Protein Sequence
MTLPKIKQVR AWFTGGATAE KGAGGGDYHD QGANHWIDDH IATPMSKYRD YEQSRQSFGI 60
NVLGTLVVEV EAENGQTGFA VSTAGEMGCF IVEKHLNRFI EGKCVSDIKL IHDQMLSATL 120
YYSGSGGLVM NTISCVDLAL WDLFGKVVGL PVYKLLGGAV RDEIQFYATG ARPDLAKEMG 180
FIGGKMPTHW GPHDGDAGIR KDAAMVADMR EKCGEDFWLM LDCWMSQDVN YATKLAHACA 240
PYNLKWIEEC LPPQQYESYR ELKRNAPVGM MVTSGEHHGT LQSFRTLSET GIDIMQPDVG 300
WCGGLTTLVE IAAIAKSRGQ LVVPHGSSVY SHHAVITFTN TPFSEFLMTS PDCSTMRPQF 360
DPILLNEPVP VNGRIHKSVL DKPGFGVELN RDCNLKRPYS H 401 
Gene Ontology
 GO:0050032; F:L-rhamnonate dehydratase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0009063; P:cellular amino acid catabolic process; IEA:InterPro. 
Interpro
 IPR023444; L-Rhamnon_dehydrat.
 IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
 IPR013342; Mandelate_racemase_C.
 IPR013341; Mandelate_racemase_N.
 IPR001354; MR_MLE. 
Pfam
 PF01188; MR_MLE
 PF02746; MR_MLE_N 
SMART
 SM00922; MR_MLE 
PROSITE
 PS00908; MR_MLE_1
 PS00909; MR_MLE_2 
PRINTS