CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 NAD(P) transhydrogenase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Nnt 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
70KEIFQNEKRVALSPAacetylation[1, 2, 3]
70KEIFQNEKRVALSPAsuccinylation[3]
84AGVQALVKQGFNVVVacetylation[4]
100SGAGEASKFPDDLYRacetylation[2]
117GAQIQGMKEVLASDLacetylation[1, 2, 3]
117GAQIQGMKEVLASDLsuccinylation[3]
171LNKLSERKTTVLAMDacetylation[3]
171LNKLSERKTTVLAMDsuccinylation[3]
224GQITAAGKVPPAKILacetylation[3]
224GQITAAGKVPPAKILsuccinylation[3]
229AGKVPPAKILIVGGGacetylation[2]
267AAALEQFKSLGAEPLubiquitination[5]
279EPLEVDLKESGEGQGubiquitination[5]
290EGQGGYAKEMSKEFIacetylation[2]
294GYAKEMSKEFIEAEMacetylation[2, 3]
294GYAKEMSKEFIEAEMsuccinylation[3]
517GKLQGILKSAPLLLPacetylation[4]
517GKLQGILKSAPLLLPubiquitination[5]
Reference
 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 713 AA 
Protein Sequence
MAHLLKTVVA GCSCPFLSNL GSSKVLPGKR DFVRTLRTHQ ALWCKSPVKP GIPYKQLTVG 60
VPKEIFQNEK RVALSPAGVQ ALVKQGFNVV VESGAGEASK FPDDLYRAAG AQIQGMKEVL 120
ASDLVVKVRA PMVNPTLGAH EADFLKPSGT LISFIYPAQN PDLLNKLSER KTTVLAMDQV 180
PRVTIAQGYD ALSSMANISG YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL 240
ASAGAAKSMG AVVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE 300
MKLFAQQCKE VDILISTALI PGGFLVTQRM LDMFKRPTDP PEYNYLYLLP GGTFVGGYLA 360
ALYGGYNIEE IMYLGSGLCC VGALGGLSTQ GTARLGNALG MIGVAGGLAA TLGGLKPDPQ 420
LLAQMSGAMA MGGTIGLTIA KRIQISDLPQ LVAAFHSLVG LAAVLTCMAE YIVEYPHFAM 480
DATSNFTKIV AYLGTYIGGV TFSGSLVAYG KLQGILKSAP LLLPGRHALN AGLLAASVGG 540
IIPFMADPSF TTGITCLGSV SALSTLMGVT LTAAIGGADM PVVITVLNSY SGWALCAEGF 600
LLNNNLLTIV GALIGSSGAI LSYIMCVAMN RSLANVILGG YGTTSTAGGK PMEISGTHTE 660
INLDNAVEMI REANSIVITP GYGLCAAKAQ YPIADLVKML TEQGKKVRLV RCV 713 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0008750; F:NAD(P)+ transhydrogenase (AB-specific) activity; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro. 
Interpro
 IPR008142; Ala_DH/PNT_CS1.
 IPR008143; Ala_DH/PNT_CS2.
 IPR007886; AlaDH/PNT_N.
 IPR007698; AlaDH/PNT_NAD(H)-bd.
 IPR016040; NAD(P)-bd_dom.
 IPR012136; NADH_DH_b. 
Pfam
 PF01262; AlaDh_PNT_C
 PF05222; AlaDh_PNT_N
 PF02233; PNTB 
SMART
 SM01002; AlaDh_PNT_C
 SM01003; AlaDh_PNT_N 
PROSITE
 PS00836; ALADH_PNT_1
 PS00837; ALADH_PNT_2 
PRINTS