CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030546
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tetratricopeptide repeat domain 8, isoform CRA_a 
Protein Synonyms/Alias
 Tetratricopeptide repeat protein 8; cDNA FLJ36572 fis, clone TRACH2012298, highly similar to Tetratricopeptide repeat protein 8 
Gene Name
 TTC8 
Gene Synonyms/Alias
 hCG_21081 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14KQFKSALKQQEMVDTubiquitination[1, 2, 3, 4]
43VTALNLFKQGLDKFPubiquitination[1, 4]
77SSAAEYYKEVLKQDNubiquitination[1, 4]
238NFATISDKIGDLQRSubiquitination[1, 2, 3]
251RSYVAAQKSEAAFPDubiquitination[1, 2, 3, 5]
267VDTQHLIKQLRQHFAubiquitination[3, 5, 6, 7]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 276 AA 
Protein Sequence
MYREAEKQFK SALKQQEMVD TFLYLAKVYV SLDQPVTALN LFKQGLDKFP GEVTLLCGIA 60
RIYEEMNNMS SAAEYYKEVL KQDNTHVEAI ACIGSNHFYS DQPEIALRFY RRLLQMGIYN 120
GQLFNNLGLC CFYAQQYDMT LTSFERALSL AENEEEAADV WYNLGHVAVG IGDTNLAHQC 180
FRLALVNNNN HAEAYNNLAV LEMRKGHVEQ ARALLQTASS LAPHMYEPHF NFATISDKIG 240
DLQRSYVAAQ KSEAAFPDHV DTQHLIKQLR QHFAML 276 
Gene Ontology
 GO:0034464; C:BBSome; IEA:Compara.
 GO:0032391; C:photoreceptor connecting cilium; IEA:Compara.
 GO:0045444; P:fat cell differentiation; IEA:Compara. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF13181; TPR_8 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS