CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014135
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Leucine-rich repeat-containing protein 16A 
Protein Synonyms/Alias
 CARMIL homolog 
Gene Name
 LRRC16A 
Gene Synonyms/Alias
 CARMIL; LRRC16 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116HIGTCLRKIFPGLSPubiquitination[1, 2]
593MGAKMLAKALQINTKubiquitination[2]
600KALQINTKLRTVIWDubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. Increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity). 
Sequence Annotation
 REPEAT 245 269 LRR 1.
 REPEAT 275 298 LRR 2.
 REPEAT 304 327 LRR 3.
 REPEAT 336 363 LRR 4.
 REPEAT 391 418 LRR 5.
 REPEAT 423 447 LRR 6.
 REPEAT 481 506 LRR 7.
 REPEAT 543 566 LRR 8.
 REPEAT 570 593 LRR 9.
 REPEAT 654 678 LRR 10.
 REPEAT 958 981 LRR 11.
 REGION 958 1082 Inhibits capping activity of CAPZA2 (By
 MOD_RES 916 916 Phosphothreonine.
 MOD_RES 968 968 Phosphoserine.
 MOD_RES 1067 1067 Phosphoserine.
 MOD_RES 1288 1288 Phosphoserine.
 MOD_RES 1291 1291 Phosphoserine.
 MOD_RES 1315 1315 Phosphoserine.
 MOD_RES 1331 1331 Phosphoserine.
 MOD_RES 1360 1360 Phosphoserine.
 CROSSLNK 593 593 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Leucine-rich repeat; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1371 AA 
Protein Sequence
MTEESSDVPR ELIESIKDVI GRKIKISVKK KVKLEVKGDK VENKVLVLTS CRAFLVTARI 60
PTKLELTFSY LEIHGVVCSK SAQMIVETEK CSISMKMASP EDVSEVLAHI GTCLRKIFPG 120
LSPVRIMKKV SMEPSERLAS LQALWDSQTV AEQGPCGGFS QMYACVCDWL GFSYREEVQW 180
DVDTIYLTQD TRELNLQDFS HLDHRDLIPI IAALEYNQWF TKLSSKDLKL STDVCEQILR 240
VVSRSNRLEE LVLENAGLRT DFAQKLASAL AHNPNSGLHT INLAGNPLED RGVSSLSIQF 300
AKLPKGLKHL NLSKTSLSPK GVNSLSQSLS ANPLTASTLV HLDLSGNVLR GDDLSHMYNF 360
LAQPNAIVHL DLSNTECSLD MVCGALLRGC LQYLAVLNLS RTVFSHRKGK EVPPSFKQFF 420
SSSLALMHIN LSGTKLSPEP LKALLLGLAC NHNLKGVSLD LSNCELRSGG AQVLEGCIAE 480
IHNITSLDIS DNGLESDLST LIVWLSKNRS IQHLALGKNF NNMKSKNLTP VLDNLVQMIQ 540
DEESPLQSLS LADSKLKTEV TIIINALGSN TSLTKVDISG NGMGDMGAKM LAKALQINTK 600
LRTVIWDKNN ITAQGFQDIA VAMEKNYTLR FMPIPMYDAS QALKTNPEKT EDALQKIENY 660
LLRNHETRKY LQEQAYRLQQ GIVTSTTQQM IDRICVKVQD HLNSLRNCGG DAIQEDLKSA 720
ERLMRDAKNS KTLLPNLYHV GGASWAGASG LLSSPIQETL ESMAGEVTRV VDEQLKALLE 780
SMVDAAENLC PNVMKKAHIR QDLIHASTEK ISIPRTFVKN VLLEQSGIDI LNKISEVKLT 840
VASFLSDRIV DEILDALSHC HHKLADHFSR RGKTLPQQES LEIELAEEKP VKRSIITVEE 900
LTEIERLEDL DTCMMTPKSK RKSIHSRMLR PVSRAFEMEF DLDKALEEVP IHIEDPPFPS 960
LRQEKRSSGF ISELPSEEGK KLEHFTKLRP KRNKKQQPTQ AAVCAANIVS QDGEQNGLMG 1020
RVDEGVDEFF TKKVTKMDSK KWSTRGSESH ELNEGGDEKK KRDSRKSSGF LNLIKSRSKS 1080
ERPPTILMTE EPSSPKGAVR SPPVDCPRKD TKAAEHNGNS ERIEEIKTPD SFEESQGEEI 1140
GKVERSDSKS SPQAGRRYGV QVMGSGLLAE MKAKQEKRAA CAQKKLGNDA VSQDSSSPAL 1200
SGVERSDGGG AVPKLHPGLP ENRFGLGTPE KNTKAEPKAE AGSRSRSSSS TPTSPKPLLQ 1260
SPKPSLAARP VIPQKPRTAS RPDDIPDSPS SPKVALLPPV LKKVPSDKER DGQSSPQPSP 1320
RTFSQEVSRR SWGQQAQEYQ EQKQRSSSKD GHQGSKSNDS GEEAEKEFIF V 1371 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0007015; P:actin filament organization; NAS:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0016477; P:cell migration; IMP:BHF-UCL.
 GO:0030032; P:lamellipodium assembly; IMP:BHF-UCL.
 GO:0031529; P:ruffle organization; IMP:BHF-UCL.
 GO:0046415; P:urate metabolic process; IMP:BHF-UCL. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS