CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001392
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 
Protein Synonyms/Alias
 Brain cyclic nucleotide-gated channel 1; BCNG-1; Hyperpolarization-activated cation channel 2; HAC-2 
Gene Name
 Hcn1 
Gene Synonyms/Alias
 Bcng1; Hac2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
97MLQPGVNKFSLRMFGubiquitination[1]
419LPADMRQKIHDYYEHubiquitination[1]
535GEICLLTKGRRTASVubiquitination[1]
586RLDRIGKKNSILLQKubiquitination[1]
593KNSILLQKFQKDLNTubiquitination[1]
596ILLQKFQKDLNTGVFubiquitination[1]
612NQENEILKQIVKHDRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. 
Sequence Annotation
 NP_BIND 464 581 cAMP.
 REGION 78 129 Involved in subunit assembly (By
 MOD_RES 39 39 Phosphothreonine.
 MOD_RES 69 69 Phosphoserine.
 CARBOHYD 327 327 N-linked (GlcNAc...) (Probable).  
Keyword
 3D-structure; cAMP; cAMP-binding; Complete proteome; Glycoprotein; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Nucleotide-binding; Phosphoprotein; Potassium; Potassium channel; Potassium transport; Reference proteome; Sodium; Sodium channel; Sodium transport; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MEGGGKPNSA SNSRDDGNSV FPSKAPATGP VAADKRLGTP PGGGAAGKEH GNSVCFKVDG 60
GGGEEPAGSF EDAEGPRRQY GFMQRQFTSM LQPGVNKFSL RMFGSQKAVE KEQERVKTAG 120
FWIIHPYSDF RFYWDLIMLI MMVGNLVIIP VGITFFTEQT TTPWIIFNVA SDTVFLLDLI 180
MNFRTGTVNE DSSEIILDPK VIKMNYLKSW FVVDFISSIP VDYIFLIVEK GMDSEVYKTA 240
RALRIVRFTK ILSLLRLLRL SRLIRYIHQW EEIFHMTYDL ASAVVRIFNL IGMMLLLCHW 300
DGCLQFLVPL LQDFPPDCWV SLNEMVNDSW GKQYSYALFK AMSHMLCIGY GAQAPVSMSD 360
LWITMLSMIV GATCYAMFVG HATALIQSLD SSRRQYQEKY KQVEQYMSFH KLPADMRQKI 420
HDYYEHRYQG KIFDEENILS ELNDPLREEI VNFNCRKLVA TMPLFANADP NFVTAMLSKL 480
RFEVFQPGDY IIREGAVGKK MYFIQHGVAG VITKSSKEMK LTDGSYFGEI CLLTKGRRTA 540
SVRADTYCRL YSLSVDNFNE VLEEYPMMRR AFETVAIDRL DRIGKKNSIL LQKFQKDLNT 600
GVFNNQENEI LKQIVKHDRE MVQAIPPINY PQMTALNCTS STTTPTSRMR TQSPPVYTAT 660
SLSHSNLHSP SPSTQTPQPS AILSPCSYTT AVCSPPIQSP LATRTFHYAS PTASQLSLMQ 720
QPQQQLPQSQ VQQTQTQTQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQPQTPG 780
SSTPKNEVHK STQALHNTNL TKEVRPLSAS QPSLPHEVST LISRPHPTVG ESLASIPQPV 840
AAVHSTGLQA GSRSTVPQRV TLFRQMSSGA IPPNRGVPPA PPPPAAVQRE SPSVLNTDPD 900
AEKPRFASNL 910 
Gene Ontology
 GO:0030424; C:axon; IDA:MGI.
 GO:0030425; C:dendrite; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0005272; F:sodium channel activity; IEA:UniProtKB-KW.
 GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
 GO:0045176; P:apical protein localization; IMP:MGI.
 GO:0046549; P:retinal cone cell development; IMP:MGI.
 GO:0035725; P:sodium ion transmembrane transport; IEA:GOC. 
Interpro
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR005821; Ion_trans_dom.
 IPR013621; Ion_trans_N.
 IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF00520; Ion_trans
 PF08412; Ion_trans_N 
SMART
 SM00100; cNMP 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 
PRINTS
 PR01463; EAGCHANLFMLY.