CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017083
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch repeat and BTB domain-containing protein 2 
Protein Synonyms/Alias
 BTB and kelch domain-containing protein 1 
Gene Name
 KBTBD2 
Gene Synonyms/Alias
 BKLHD1; KIAA1489; CGI-73 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
127CREYLIKKINAENCVubiquitination[1]
149LFSCEELKQSAKRMVubiquitination[1, 2]
251VVVQGLYKSMPKFFKubiquitination[1, 3, 4]
297PQAEKVYKLCSPPADubiquitination[1]
307SPPADLHKVGTVVTPubiquitination[1]
328AGGQVPLKNTKTNHSubiquitination[2, 5, 6, 7]
331QVPLKNTKTNHSKTSubiquitination[1]
339TNHSKTSKLQTAFRTubiquitination[1]
518MAANIPAKRYSDPCVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
 DOMAIN 31 98 BTB.
 REPEAT 317 380 Kelch 1.
 REPEAT 381 429 Kelch 2.
 REPEAT 431 469 Kelch 3.
 REPEAT 470 529 Kelch 4.
 REPEAT 535 581 Kelch 5.
 MOD_RES 300 300 Phosphoserine.
 MOD_RES 310 310 Phosphothreonine.
 MOD_RES 313 313 Phosphothreonine.
 MOD_RES 319 319 Phosphotyrosine.  
Keyword
 Complete proteome; Kelch repeat; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 623 AA 
Protein Sequence
MSTQDERQIN TEYAVSLLEQ LKLFYEQQLF TDIVLIVEGT EFPCHKMVLA TCSSYFRAMF 60
MSGLSESKQT HVHLRNVDAA TLQIIITYAY TGNLAMNDST VEQLYETACF LQVEDVLQRC 120
REYLIKKINA ENCVRLLSFA DLFSCEELKQ SAKRMVEHKF TAVYHQDAFM QLSHDLLIDI 180
LSSDNLNVEK EETVREAAML WLEYNTESRS QYLSSVLSQI RIDALSEVTQ RAWFQGLPPN 240
DKSVVVQGLY KSMPKFFKPR LGMTKEEMMI FIEASSENPC SLYSSVCYSP QAEKVYKLCS 300
PPADLHKVGT VVTPDNDIYI AGGQVPLKNT KTNHSKTSKL QTAFRTVNCF YWFDAQQNTW 360
FPKTPMLFVR IKPSLVCCEG YIYAIGGDSV GGELNRRTVE RYDTEKDEWT MVSPLPCAWQ 420
WSAAVVVHDC IYVMTLNLMY CYFPRSDSWV EMAMRQTSRS FASAAAFGDK IFYIGGLHIA 480
TNSGIRLPSG TVDGSSVTVE IYDVNKNEWK MAANIPAKRY SDPCVRAVVI SNSLCVFMRE 540
THLNERAKYV TYQYDLELDR WSLRQHISER VLWDLGRDFR CTVGKLYPSC LEESPWKPPT 600
YLFSTDGTEE FELDGEMVAL PPV 623 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS