CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023839
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Meiosis arrest female protein 1 
Protein Synonyms/Alias
 Limkain-b1 
Gene Name
 KIAA0430 
Gene Synonyms/Alias
 LKAP; MARF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25LQQDNDAKPWLWKFSubiquitination[1]
73SPLHAGSKLFPAVPLubiquitination[1]
90IRSLQQPKIQLSSVPubiquitination[1]
189ESNLSSCKHLPCCGKubiquitination[1]
196KHLPCCGKLHFQSCHubiquitination[1]
277YCEDCLNKPARNSIIubiquitination[1, 2]
288NSIIDAAKVWPNIPPubiquitination[1]
318CGTKGTGKETTLLLAubiquitination[1]
330LLATSLGKAASKFGSubiquitination[1]
380VVQRIREKFFKGHREubiquitination[1]
401CDISKENKEVIQELNubiquitination[1]
422AHINATAKNAADDKLubiquitination[1]
542LSDNCGGKVLSITGCubiquitination[1]
585IIVSFTPKNRELCETubiquitination[1]
593NRELCETKSSNAIADubiquitination[1]
617NPKLCLIKDASEQSSubiquitination[1]
648ATKNTNVKSLQELCRubiquitination[1]
690SAAVSTPKNSGVAEPubiquitination[1]
700GVAEPVYKTSQKKENubiquitination[1]
738SYPSAFSKLVASRQVubiquitination[1]
805IDYRLSRKELQQLLQubiquitination[1]
822FARHGKVKSVELSPHubiquitination[1]
919LNVSDLYKLTDTVAIubiquitination[1]
1193LLKSQASKQVIVREFubiquitination[1]
1265ERTKQFSKDVVDLLRubiquitination[1]
1388SISALTQKLCHVVKVubiquitination[1]
1394QKLCHVVKVADIESGubiquitination[1]
1558HKRIVVLKNDMKSRLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Essential regulator of oogenesis required for female meiotic progression. Acts by down-regulating RNA transcripts, either at transcriptional of post-transcriptional level (By similarity). 
Sequence Annotation
 DOMAIN 353 490 NYN.
 DOMAIN 791 870 RRM.
 DOMAIN 875 949 HTH OST-type 1.
 DOMAIN 1003 1079 HTH OST-type 2.
 DOMAIN 1099 1173 HTH OST-type 3.
 DOMAIN 1175 1250 HTH OST-type 4.
 DOMAIN 1259 1334 HTH OST-type 5.
 DOMAIN 1335 1410 HTH OST-type 6.
 DOMAIN 1411 1485 HTH OST-type 7.
 DOMAIN 1486 1560 HTH OST-type 8.
 MOD_RES 699 699 Phosphotyrosine (By similarity).
 MOD_RES 1091 1091 Phosphoserine.
 MOD_RES 1093 1093 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Differentiation; Meiosis; Oogenesis; Peroxisome; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1742 AA 
Protein Sequence
MMEGNGTENS CSRTRGWLQQ DNDAKPWLWK FSNCFSRPEQ TLPHSPQTKE YMENKKVAVE 60
LKDVPSPLHA GSKLFPAVPL PDIRSLQQPK IQLSSVPKVS CCAHCPNEPS TSPMRFGGGG 120
GGSGGTSSLI HPGALLDSQS TRTITCQVGS GFAFQSASSL QNASARNNLA GIASDFPSMC 180
LESNLSSCKH LPCCGKLHFQ SCHGNVHKLH QFPSLQGCTS AGYFPCSDFT SGAPGHLEEH 240
ISQSELTPHL CTNSLHLNVV PPVCLKGSLY CEDCLNKPAR NSIIDAAKVW PNIPPPNTQP 300
APLAVPLCNG CGTKGTGKET TLLLATSLGK AASKFGSPEV AVAGQVLENL PPIGVFWDIE 360
NCSVPSGRSA TAVVQRIREK FFKGHREAEF ICVCDISKEN KEVIQELNNC QVTVAHINAT 420
AKNAADDKLR QSLRRFANTH TAPATVVLVS TDVNFALELS DLRHRHGFHI ILVHKNQASE 480
ALLHHANELI RFEEFISDLP PRLPLKMPQC HTLLYVYNLP ANKDGKSVSN RLRRLSDNCG 540
GKVLSITGCS AILRFINQDS AERAQKRMEN EDVFGNRIIV SFTPKNRELC ETKSSNAIAD 600
KVKSPKKLKN PKLCLIKDAS EQSSSAKATP GKGSQANSGS ATKNTNVKSL QELCRMESKT 660
GHRNSEHQQG HLRLVVPTHG NSSAAVSTPK NSGVAEPVYK TSQKKENLSA RSVTSSPVEK 720
KDKEETVFQV SYPSAFSKLV ASRQVSPLLA SQSWSSRSMS PNLLNRASPL AFNIANSSSE 780
ADCPDPFANG ADVQVSNIDY RLSRKELQQL LQEAFARHGK VKSVELSPHT DYQLKAVVQM 840
ENLQDAIGAV NSLHRYKIGS KKILVSLATG AASKSLSLLS AETMSVLQDA PACCLPLFKF 900
TDIYEKKFGH KLNVSDLYKL TDTVAIREQG NGRLVCLLPS SQARQSPLGS SQSHDGSSTN 960
CSPIIFEELE YHEPVCRQHC SNKDFSEHEF DPDSYKIPFV ILSLKTFAPQ VHSLLQTHEG 1020
TVPLLSFPDC YIAEFGDLEV VQENQGGVPL EHFITCVPGV NIATAQNGIK VVKWIHNKPP 1080
PPNTDPWLLR SKSPVGNPQL IQFSREVIDL LKSQPSCVIP ISHFIPSYHH HFAKQCRVSD 1140
YGYSKLIELL EAVPHVLQIL GMGSKRLLTL THRAQVKRFT QDLLKLLKSQ ASKQVIVREF 1200
SQAYHWCFSK DWDVTEYGVC ELIDIVSEIP DTTICLSQQD NEMVICIPKR ERTQDEIERT 1260
KQFSKDVVDL LRHQPHFRMP FNKFIPSYHH HFGRQCKLAY YGFTKLLELF EAIPDTLQVL 1320
ECGEEKILTL TEVERFKALA AQFVKLLRSQ KDNCLMMTDL LTEYAKTFGY TFRLQDYDVS 1380
SISALTQKLC HVVKVADIES GRQIQLINRK SLRSLTAQLL VLLMSWEGTT HLSVEELKRH 1440
YESTHNTPLN PCEYGFMTLT ELLKSLPYLV EVFTNDKMEE CVKLTSLYLF AKNVRSLLHT 1500
YHYQQIFLHE FSMAYTKYVG ETLQPKTYGH SSVEELLGAI PQVVWIKGHG HKRIVVLKND 1560
MKSRLSSLSL SPANHENQPS EGERILEVPE SHTASELKLG ADGSGPSHTE QELLRLTDDS 1620
PVDLLCAPVP SCLPSPQLRP DPVILQSADL IQFEERPQEP SEIMILNQEE KMEIPIPGKS 1680
KTLTSDSSSS CISAAVPVPP CPSSETSESL LSKDPVESPA KKQPKNRVKL AANFSLAPIT 1740
KL 1742 
Gene Ontology
 GO:0005777; C:peroxisome; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007143; P:female meiosis; ISS:UniProtKB.
 GO:0010923; P:negative regulation of phosphatase activity; IDA:UniProtKB.
 GO:0048477; P:oogenesis; ISS:UniProtKB.
 GO:0010468; P:regulation of gene expression; ISS:UniProtKB. 
Interpro
 IPR024768; Limkain-b1.
 IPR024582; Limkain_b1_cons_dom.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR021139; NYN_limkain-b1.
 IPR025605; OST-HTH/LOTUS_dom.
 IPR000504; RRM_dom. 
Pfam
 PF11608; Limkain-b1
 PF01936; NYN
 PF12872; OST-HTH 
SMART
 SM00360; RRM 
PROSITE
 PS51644; HTH_OST
 PS50102; RRM 
PRINTS