CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000145
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Levanbiose-producing levanase 
Protein Synonyms/Alias
 2,6-beta-fructan 6-levanbiohydrolase; Endo-levanase 
Gene Name
 levB 
Gene Synonyms/Alias
 yveB; BSU34460 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
61KWKNDPQKPIYFDGKacetylation[1]
124TGSVVVDKENTAGFGacetylation[1]
145IVTQPSAKDKKQEQYacetylation[1]
486FSNEVFPKPEDKGITacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065
Functional Description
 Catalyzes the degradation of levan mainly into levanbiose (difructose). Is not active on sucrose. 
Sequence Annotation
 REGION 55 58 Substrate binding (By similarity).
 REGION 116 117 Substrate binding (By similarity).
 REGION 181 182 Substrate binding (By similarity).
 ACT_SITE 58 58 By similarity.
 BINDING 230 230 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).  
Keyword
 Carbohydrate metabolism; Cell membrane; Complete proteome; Glycosidase; Hydrolase; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 516 AA 
Protein Sequence
MNYIKAGKWL TVFLTFLGIL LFIDLFPKEE HDQKTKSKQK PDYRAAYHFT TPDKWKNDPQ 60
KPIYFDGKYH YFYLYNRDYP KGNGTEWRHA VSEDLVHWTD EGVAIPKYTN PDGDIWTGSV 120
VVDKENTAGF GKNALVAIVT QPSAKDKKQE QYLWYSTDKG KSFKFYSGNP VMPNPGTDDF 180
RDPKVIWDDQ DNKWVMVMAE GSKIGFYESD NLKDWHYTSG FFPEQAGMVE CPDLYMMRAS 240
DGTNKWVLGA SANGKPWGKP NTYAYWTGSF DGKEFKADQT EAQWLDYGFD WYGGVTFEDS 300
KSTDPLEKRY ALAWMNNWDY ANNTPTMKNG FNGTDSVIRE LRLKEQDGTY SLVSQPIEAL 360
EQLTVSTDEI EDQDVNGSKT LSITGDTYQL DTDLSWSELK NAGVRLRESE DQKRHIDVGI 420
FAEGGYAYVN RAATNQPDKS NTYVESKAPY DVNKRKVHLK ILVDKTTIEV FVGDGKTVFS 480
NEVFPKPEDK GITLYSDGGT ASFKNITVKH FDSIHE 516 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0033912; F:2,6-beta-fructan 6-levanbiohydrolase activity; IEA:EC.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR008985; ConA-like_lec_gl_sf.
 IPR001362; Glyco_hydro_32.
 IPR013189; Glyco_hydro_32_C.
 IPR013148; Glyco_hydro_32_N.
 IPR023296; Glyco_hydro_beta-prop. 
Pfam
 PF08244; Glyco_hydro_32C
 PF00251; Glyco_hydro_32N 
SMART
 SM00640; Glyco_32 
PROSITE
 PS00609; GLYCOSYL_HYDROL_F32 
PRINTS