UniProt Accession

 PHO4_YEAST; P07270; D6VTR7 

Genbank Protein ID

 X03719; D50617; AY692776; BK006940 

Genbank Nucleotide ID

 CAA27345.1; BAA09273.1; AAT92795.1; DAA12477.1 

Protein Name

 Phosphate system positive regulatory protein PHO4 

Protein Synonyms/Alias

  

Gene Name

 PHO4 

Gene Synonyms/Alias

 YFR034C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
157	NSSPYLNKRRGKPGP	ubiquitination	[1]
292	NVSAAPSKATTVEAA	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Transcriptional activator that regulates the expression of repressible phosphatase under phosphate starvation conditions. Binds to the upstream activating sequence (UAS) of several phosphatase encoding PHO genes. Inhibited by the cyclin-CDK PHO80- PHO85 under high-phosphate conditions. 

Sequence Annotation

 DOMAIN 250 306 bHLH.
 REGION 1 31 Interaction with PHO80.
 REGION 75 99 Transcription activation domain.
 REGION 156 200 Interaction with PHO80.
 REGION 201 218 Interaction with PHO2.
 REGION 203 227 Involved in oligomerization.
 MOTIF 75 83 9aaTAD.
 MOTIF 140 166 Nuclear localization signal.
 MOD_RES 100 100 Phosphoserine; by PHO85.
 MOD_RES 114 114 Phosphoserine; by PHO85.
 MOD_RES 128 128 Phosphoserine; by PHO85.
 MOD_RES 152 152 Phosphoserine; by PHO85.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 223 223 Phosphoserine; by PHO85.
 MOD_RES 242 242 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.  

Keyword

 3D-structure; Activator; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:SGD.
 GO:0016036; P:cellular response to phosphate starvation; IMP:SGD.
 GO:0006338; P:chromatin remodeling; IMP:SGD.
 GO:0006796; P:phosphate-containing compound metabolic process; TAS:SGD.
 GO:0045937; P:positive regulation of phosphate metabolic process; IGI:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IGI:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS