CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018164
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WAS protein family homolog 1 
Protein Synonyms/Alias
  
Gene Name
 Wash1 
Gene Synonyms/Alias
 Orf19; Wash 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
185TTENLYKKYVFLDPLacetylation[1]
198PLAGAVTKTHTMLGTubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. 
Sequence Annotation
 DOMAIN 369 391 WH2.
 REGION 1 167 WHD1.  
Keyword
 Actin-binding; Complete proteome; Endosome; Membrane; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 475 AA 
Protein Sequence
MTPVKTQCSL AGQLYAVPLI QPDLRREEAI QQVADALQYL QNISGDIFSR ISRRVELSRR 60
QLQAISERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPEHL QEYGSIFTGA LDPGLQRRPR 120
YRIQSKHRPL DERALQEKLK YFPVCVNTKS EPEDEAEEGL GGLPSNISSI SSLLLFNTTE 180
NLYKKYVFLD PLAGAVTKTH TMLGTEEEKL FDAPLSISKR EQLERQAPEN YFYVPDLGQV 240
PEIDVPSYLP DLPGVADDLM YSADLGPGIA PSAPGAIPEL PAFHTEVAEP LQPELENEVL 300
LAAPPPPPPP PPPPPPAPTA LVSTPQPPMF PDMATAAGQV AREEDSSSSM AHTASVQGAP 360
KEVVDPSSGR ATLLESIRQA GGIGKAKLRS VKERKLEKKK QKEQEQVRAT SQGGDLMSDL 420
FNKLVMRRKG ISGKGPSTGT SEGPGGAFSR MSDSIPPLPP PQQPAGDEDE EDWES 475 
Gene Ontology
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; IDA:UniProtKB.
 GO:0055038; C:recycling endosome membrane; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; ISS:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; ISS:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IMP:UniProtKB.
 GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB. 
Interpro
 IPR021854; WASH_WASD.
 IPR003124; WH2_dom. 
Pfam
 PF11945; WASH_WAHD 
SMART
  
PROSITE
 PS51082; WH2 
PRINTS