CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021610
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intraflagellar transport protein 122 homolog 
Protein Synonyms/Alias
 WD repeat-containing protein 10; WD repeat-containing protein 140 
Gene Name
 IFT122 
Gene Synonyms/Alias
 SPG; WDR10; WDR140 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50GTLLQPLKGHKDTVYubiquitination[1]
663GLDFETAKKAFIRVQubiquitination[1]
753ETKMLITKQADWARNubiquitination[1, 2]
817LCATYLKKLDSPGYAubiquitination[2]
830YAAETYLKMGDLKSLubiquitination[1]
1021LARHAYDKLRGLYIPacetylation[3]
1033YIPARFQKSIELGTLubiquitination[2, 4]
1141ILRLVETKDSIGDEDubiquitination[1, 2]
1153DEDPFTAKLSFEQGGubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Required for cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity). 
Sequence Annotation
 REPEAT 10 50 WD 1.
 REPEAT 51 91 WD 2.
 REPEAT 93 129 WD 3.
 REPEAT 131 169 WD 4.
 REPEAT 278 317 WD 5.
 REPEAT 319 359 WD 6.
 REPEAT 512 551 WD 7.  
Keyword
 Alternative splicing; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Disease mutation; Ectodermal dysplasia; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1241 AA 
Protein Sequence
MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA 60
YAKDGKRFAS GSADKSVIIW TSKLEGILKY THNDAIQCVS YNPITHQLAS CSSSDFGLWS 120
PEQKSVSKHK SSSKIICCSW TNDGQYLALG MFNGIISIRN KNGEEKVKIE RPGGSLSPIW 180
SICWNPSSRW ESFWMNRENE DAEDVIVNRY IQEIPSTLKS AVYSSQGSEA EEEEPEEEDD 240
SPRDDNLEER NDILAVADWG QKVSFYQLSG KQIGKDRALN FDPCCISYFT KGEYILLGGS 300
DKQVSLFTKD GVRLGTVGEQ NSWVWTCQAK PDSNYVVVGC QDGTISFYQL IFSTVHGLYK 360
DRYAYRDSMT DVIVQHLITE QKVRIKCKEL VKKIAIYRNR LAIQLPEKIL IYELYSEDLS 420
DMHYRVKEKI IKKFECNLLV VCANHIILCQ EKRLQCLSFS GVKEREWQME SLIRYIKVIG 480
GPPGREGLLV GLKNGQILKI FVDNLFAIVL LKQATAVRCL DMSASRKKLA VVDENDTCLV 540
YDIDTKELLF QEPNANSVAW NTQCEDMLCF SGGGYLNIKA STFPVHRQKL QGFVVGYNGS 600
KIFCLHVFSI SAVEVPQSAP MYQYLDRKLF KEAYQIACLG VTDTDWRELA MEALEGLDFE 660
TAKKAFIRVQ DLRYLELISS IEERKKRGET NNDLFLADVF SYQGKFHEAA KLYKRSGHEN 720
LALEMYTDLC MFEYAKDFLG SGDPKETKML ITKQADWARN IKEPKAAVEM YISAGEHVKA 780
IEICGDHGWV DMLIDIARKL DKAEREPLLL CATYLKKLDS PGYAAETYLK MGDLKSLVQL 840
HVETQRWDEA FALGEKHPEF KDDIYMPYAQ WLAENDRFEE AQKAFHKAGR QREAVQVLEQ 900
LTNNAVAESR FNDAAYYYWM LSMQCLDIAQ DPAQKDTMLG KFYHFQRLAE LYHGYHAIHR 960
HTEDPFSVHR PETLFNISRF LLHSLPKDTP SGISKVKILF TLAKQSKALG AYRLARHAYD 1020
KLRGLYIPAR FQKSIELGTL TIRAKPFHDS EELVPLCYRC STNNPLLNNL GNVCINCRQP 1080
FIFSASSYDV LHLVEFYLEE GITDEEAISL IDLEVLRPKR DDRQLEIANN SSQILRLVET 1140
KDSIGDEDPF TAKLSFEQGG SEFVPVVVSR LVLRSMSRRD VLIKRWPPPL RWQYFRSLLP 1200
DASITMCPSC FQMFHSEDYE LLVLQHGCCP YCRRCKDDPG P 1241 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0030991; C:intraflagellar transport particle A; IDA:UniProtKB.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0032391; C:photoreceptor connecting cilium; ISS:UniProtKB.
 GO:0048593; P:camera-type eye morphogenesis; ISS:UniProtKB.
 GO:0060830; P:ciliary receptor clustering involved in smoothened signaling pathway; IEA:Compara.
 GO:0060271; P:cilium morphogenesis; ISS:UniProtKB.
 GO:0010172; P:embryonic body morphogenesis; ISS:UniProtKB.
 GO:0042733; P:embryonic digit morphogenesis; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; ISS:UniProtKB.
 GO:0035721; P:intraflagellar retrograde transport; ISS:UniProtKB.
 GO:0060173; P:limb development; ISS:UniProtKB.
 GO:0045879; P:negative regulation of smoothened signaling pathway; ISS:UniProtKB.
 GO:0001843; P:neural tube closure; ISS:UniProtKB.
 GO:0060831; P:smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IEA:Compara. 
Interpro
 IPR011990; TPR-like_helical.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS