CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018575
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 35 
Protein Synonyms/Alias
 Glucocorticoid receptor DNA-binding factor 1 
Gene Name
 Arhgap35 
Gene Synonyms/Alias
 Grlf1; Kiaa1722 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
368LSCIKAKKLLETKPEubiquitination[1]
625ALCTNDDKYVIDGKMacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor (By similarity). 
Sequence Annotation
 DOMAIN 270 327 FF 1.
 DOMAIN 368 422 FF 2.
 DOMAIN 429 483 FF 3.
 DOMAIN 485 550 FF 4.
 DOMAIN 1249 1436 Rho-GAP.
 MOD_RES 308 308 Phosphotyrosine (By similarity).
 MOD_RES 589 589 Phosphoserine (By similarity).
 MOD_RES 773 773 Phosphoserine.
 MOD_RES 970 970 Phosphoserine (By similarity).
 MOD_RES 975 975 Phosphoserine (By similarity).
 MOD_RES 980 980 Phosphoserine.
 MOD_RES 1072 1072 Phosphoserine (By similarity).
 MOD_RES 1087 1087 Phosphotyrosine.
 MOD_RES 1105 1105 Phosphotyrosine; by ABL2 and PTK6.
 MOD_RES 1134 1134 Phosphoserine (By similarity).
 MOD_RES 1150 1150 Phosphoserine (By similarity).
 MOD_RES 1179 1179 Phosphoserine (By similarity).  
Keyword
 Complete proteome; DNA-binding; GTPase activation; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1499 AA 
Protein Sequence
MMMARKQDVR IPTYNISVVG LSGTEKEKGQ CGIGKSCLCN RFVRPSADEF HLDHTSVLST 60
SDFGGRVVNN DHFLYWGEVS RSLEDCVECK MHIVEQTEFI DDQTFQPHRS TALQPYIKRA 120
AATKLASAEK LMYFCTDQLG LEQDFEQKQM PDGKLLVDGF LLGIDVSRGM NRNFDDQLKF 180
VSNLYNQLAK TKKPIVVVLT KCDEGVERYI RDAHTFALSK KNLQVVETSA RSNVNVDLAF 240
STLVQLIDKS RGKTKIIPYF EALKQQSQQI ATAKDKYEWL VSRIVKNHNE NWPSVSRKMQ 300
ASPEYQDYVY LEGTQKAKKL FLQHIHRLKH EHIERRRKLY LAALPLAFEA LIPNLDEVDH 360
LSCIKAKKLL ETKPEFLKWF VVLEETPWDA TSHIDNMENE RIPFDLMDTV PAEQLYETHL 420
EKLRNERKRA EMRRAFKENL ETSPFITPGK PWEEARSFIM NEDFYQWLEE SVYMDIYGKH 480
QKQIIDRAKE EFQELLLEYS ELFYELELDA KPSKEKMGVI QDVLGEEQRF KALQKLQAER 540
DALILKHIHF VYHPTKETCP SCPACVDAKI EHLISSRFIR PSDRNQKNSL SDLNIDRINL 600
VILGKDGLAR ELANEIRALC TNDDKYVIDG KMYELSLRPI EGNVRLPVNS FQTPTFQPHG 660
CLCLYNSKES LSYVVESIEK SRESTLGRRD NHLVHLPLTL ILVNKRGDTS GETLHSLIQQ 720
GQQIASKLQC VFLDPASAGI GYGRNINEKQ ISQVLKGLLD SKRNLNLVSS TASIKDLADV 780
DLRIVMCLMC GDPFSADDVL SPVLQSQTCK SSHCGSSNSV LLELPIGLHK KRIELSVLSY 840
HSSFSIRKSR LVHGYIVFYS AKRKASLAML RAFLCEVQDI IPIQLVALTD GAIDVLDNDL 900
SREQLTEGEE IAQEIDGRFT SIPCSQPQHK LELFHPFFKD VVEKKNIIEA THMYDNVAEA 960
CSTTEEVFNS PRAGSPLCNS NLQDSEEDVE PPSYHLFRED ATLPSLSKDH SKFSMELEGN 1020
DGLSFIMSNF ESKLNNKVPP PVKPKPPVHF DITKDLSYLD QGHREGQRKS MSSSPWMPQD 1080
GFDPSDYAEP MDAVVKPRNE EENIYSVPHD STQGKIITIR NINKAQSNGS GNGSDSEMDT 1140
SSLERGRKVS AVSKPVLYRT RCTRLGRFAS YRTSFSVGSD DELGPIRKKE EDQASQGYKG 1200
DNAVIPYETD EDPRRRNILR SLRRNTKKPK PKPRPSITKA TWESNYFGVP LTTVVTPEKP 1260
IPIFIERCIE YIEATGLSTE GIYRVSGNKS EMESLQRQFD QDHNLDLAEK DFTVNTVAGA 1320
MKSFFSELPD PLVPYSMQID LVEAHKINDR EQKLHALKEV LKKFPKENHE VFKYVISHLN 1380
KVSHNNKVNL MTSENLSICF WPTLMRPDFS SMDALTATRS YQTIIELFIQ QCPFFFYNRP 1440
ISEPPGAAPG SPSAMAPTVP FLTSTPATSQ PSPPQSPPPT PQSPMQPLLS SQLQAEHTL 1499 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:MGI.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0005096; F:GTPase activator activity; TAS:MGI.
 GO:0043010; P:camera-type eye development; IMP:MGI.
 GO:0030900; P:forebrain development; IMP:MGI.
 GO:0007229; P:integrin-mediated signaling pathway; TAS:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0043116; P:negative regulation of vascular permeability; IMP:MGI.
 GO:0001843; P:neural tube closure; IMP:MGI.
 GO:0008360; P:regulation of cell shape; IDA:MGI.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR002713; FF_domain.
 IPR027417; P-loop_NTPase.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001806; Small_GTPase. 
Pfam
 PF01846; FF
 PF00071; Ras
 PF00620; RhoGAP 
SMART
 SM00441; FF
 SM00324; RhoGAP 
PROSITE
 PS51676; FF
 PS50238; RHOGAP 
PRINTS