UniProt Accession

 PAPS1_HUMAN; O43252; O43841; O75332; Q96FB1; Q96TF4; Q9P1P9; Q9UE98 

Genbank Protein ID

 Y10387; U53447; AF033026; AF016496; AF105227; AF097721; AF097710; AF097711; AF097712; AF097713; AF097714; AF097715; AF097716; AF097717; AF097718; AF097719; AF097720; BC011392; BC050627 

Genbank Nucleotide ID

 CAA71413.1; AAC39894.1; AAC28429.1; AAD09325.1; AAF40236.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAF40235.1; AAH11392.1; AAH50627.1 

Protein Name

 Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 

Protein Synonyms/Alias

 PAPS synthase 1; PAPSS 1; Sulfurylase kinase 1; SK 1; SK1; Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; Adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenylylsulfate 3'-phosphotransferase 

Gene Name

 PAPSS1 

Gene Synonyms/Alias

 ATPSK1; PAPSS 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	IPGSLCKKVKLSNNA	ubiquitination	[1]
12	GSLCKKVKLSNNAQN	ubiquitination	[2]
183	KARAGEIKGFTGIDS	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
243	ELYVPENKLHLAKTD	ubiquitination	[1, 8]
404	RLTPTELKQKFKDMN	ubiquitination	[1, 8]
406	TPTELKQKFKDMNAD	ubiquitination	[1]
619	TVLTEYYKSLEKA**	ubiquitination	[1, 2, 4, 5, 6, 7, 8, 9]
623	EYYKSLEKA******	ubiquitination	[2, 7, 9]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. 

Sequence Annotation

 NP_BIND 59 66 ATP (Potential).
 REGION 1 ?220 Adenylyl-sulfate kinase.
 REGION ?221 624 Sulfate adenylyltransferase.
 MOTIF 521 525 PP-motif.
 ACT_SITE 133 133 Phosphoserine intermediate (By  

Keyword

 3D-structure; ATP-binding; Complete proteome; Kinase; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 624 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0004020; F:adenylylsulfate kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004781; F:sulfate adenylyltransferase (ATP) activity; ISS:UniProtKB.
 GO:0050428; P:3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; TAS:Reactome.
 GO:0001501; P:skeletal system development; TAS:ProtInc.
 GO:0000103; P:sulfate assimilation; IEA:UniProtKB-UniPathway.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 

Interpro

 IPR002891; APS_kinase.
 IPR025980; ATP-Sase_PUA-like_dom.
 IPR027417; P-loop_NTPase.
 IPR015947; PUA-like_domain.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR024951; Sulfurylase_cat_dom.
 IPR002650; Sulphate_adenylyltransferase. 

Pfam

 PF01583; APS_kinase
 PF01747; ATP-sulfurylase
 PF14306; PUA_2 

SMART

  

PROSITE

  

PRINTS