CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011330
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Folylpolyglutamate synthase, mitochondrial 
Protein Synonyms/Alias
 Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase 
Gene Name
 FPGS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24AGYLEQVKRQRGDPQubiquitination[1]
206WQKGGIFKQGVPAFTubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstitued reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates. 
Sequence Annotation
 NP_BIND 103 109 ATP (Potential).  
Keyword
 Alternative initiation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Ligase; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleotide-binding; One-carbon metabolism; Polymorphism; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 587 AA 
Protein Sequence
MEYQDAVRML NTLQTNAGYL EQVKRQRGDP QTQLEAMELY LARSGLQVED LDRLNIIHVT 60
GTKGKGSTCA FTECILRSYG LKTGFFSSPH LVQVRERIRI NGQPISPELF TKYFWRLYHR 120
LEETKDGSCV SMPPYFRFLT LMAFHVFLQE KVDLAVVEVG IGGAYDCTNI IRKPVVCGVS 180
SLGIDHTSLL GDTVEKIAWQ KGGIFKQGVP AFTVLQPEGP LAVLRDRAQQ ISCPLYLCPM 240
LEALEEGGPP LTLGLEGEHQ RSNAALALQL AHCWLQRQDR HGAGEPKASR PGLLWQLPLA 300
PVFQPTSHMR LGLRNTEWPG RTQVLRRGPL TWYLDGAHTA SSAQACVRWF RQALQGRERP 360
SGGPEVRVLL FNATGDRDPA ALLKLLQPCQ FDYAVFCPNL TEVSSTGNAD QQNFTVTLDQ 420
VLLRCLEHQQ HWNHLDEEQA SPDLWSAPSP EPGGSASLLL APHPPHTCSA SSLVFSCISH 480
ALQWISQGRD PIFQPPSPPK GLLTHPVAHS GASILREAAA IHVLVTGSLH LVGGVLKLLE 540
PALSQ 545 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004326; F:tetrahydrofolylpolyglutamate synthase activity; TAS:ProtInc.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0046655; P:folic acid metabolic process; TAS:Reactome.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0042221; P:response to chemical stimulus; IEA:Compara. 
Interpro
 IPR001645; Folylpolyglutamate_synth.
 IPR018109; Folylpolyglutamate_synth_CS.
 IPR023600; Folylpolyglutamate_synth_euk.
 IPR004101; Mur_ligase_C.
 IPR013221; Mur_ligase_cen. 
Pfam
  
SMART
  
PROSITE
 PS01011; FOLYLPOLYGLU_SYNT_1
 PS01012; FOLYLPOLYGLU_SYNT_2 
PRINTS