UniProt Accession

 TRM1_HUMAN; Q9NXH9; O76103; Q548Y5; Q8WVA6 

Genbank Protein ID

 AF196479; AK000251; AC005546; BC002492; BC018302; BC040126 

Genbank Nucleotide ID

 AAG28495.1; BAA91031.1; AAC33150.1; AAH02492.1; AAH18302.1; AAH40126.1 

Protein Name

 tRNA (guanine(26)-N(2))-dimethyltransferase 

Protein Synonyms/Alias

 tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase 

Gene Name

 TRMT1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
100	ARIQLGAKGIQIKVP	ubiquitination	[1]
105	GAKGIQIKVPGEKDT	ubiquitination	[2]
130	EEEKVELKESENLAS	ubiquitination	[2]
283	KYGAMALKSRACHEM	ubiquitination	[1]
371	GVPSGRAKFSAACGP	ubiquitination	[1]
483	VSLSHACKNAVKTDA	ubiquitination	[1, 3]
487	HACKNAVKTDAPASA	ubiquitination	[1, 2, 3, 4, 5, 6]
504	DIMRCWEKECPVKRE	ubiquitination	[1]
570	PRARPGGKAADEAME	ubiquitination	[1]
588	RLLQNKRKEPPEDVA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups. 

Sequence Annotation

 ZN_FING 600 627 C3H1-type.
 MOD_RES 120 120 Phosphoserine (By similarity).
 MOD_RES 625 625 Phosphoserine.
 MOD_RES 628 628 Phosphothreonine.
 MOD_RES 646 646 Phosphothreonine.  

Keyword

 Alternative splicing; Complete proteome; Metal-binding; Methyltransferase; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 659 AA 

Protein Sequence

Gene Ontology

 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0004809; F:tRNA (guanine-N2-)-methyltransferase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 

Interpro

 IPR002905; TRM1.
 IPR000571; Znf_CCCH. 

Pfam

 PF02005; TRM
 PF00642; zf-CCCH 

SMART

 SM00356; ZnF_C3H1 

PROSITE

 PS50103; ZF_C3H1 

PRINTS