CPLM-008414

Genbank Nucleotide ID

Ubiquitin-conjugating enzyme E2 D1

Protein Synonyms/Alias

Stimulator of Fe transport; SFT; UBC4/5 homolog; UbcH5; Ubiquitin carrier protein D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1

SFT; UBC5A; UBCH5; UBCH5A

Homo sapiens (Human)

Position	Peptide	Type	References
8	MALKRIQKELSDLQR	ubiquitination	[1]
144	HAREWTQKYAM****	acetylation	[2]
144	HAREWTQKYAM****	ubiquitination	[1, 3]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

ACT_SITE 85 85 Glycyl thioester intermediate (By

3D-structure; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
GO:0030509; P:BMP signaling pathway; TAS:Reactome.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:Reactome.
GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0070936; P:protein K48-linked ubiquitination; IDA:UniProtKB.
GO:0061418; P:regulation of transcription from RNA polymerase II promoter in response to hypoxia; TAS:Reactome.
GO:0034138; P:toll-like receptor 3 signaling pathway; TAS:Reactome.
GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
GO:0035666; P:TRIF-dependent toll-like receptor signaling pathway; TAS:Reactome.

IPR000608; UBQ-conjugat_E2.
IPR023313; UBQ-conjugating_AS.
IPR016135; UBQ-conjugating_enzyme/RWD.

PS00183; UBIQUITIN_CONJUGAT_1
PS50127; UBIQUITIN_CONJUGAT_2