CPLM-008159

Genbank Nucleotide ID

Ubiquitin-conjugating enzyme E2 A

Protein Synonyms/Alias

RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A

Homo sapiens (Human)

Position	Peptide	Type	References
66	FTEEYPNKPPTVRFV	acetylation	[1]
66	FTEEYPNKPPTVRFV	ubiquitination	[2, 3, 4, 5, 6]
75	PTVRFVSKMFHPNVY	ubiquitination	[3]

[1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.

ACT_SITE 88 88 Glycyl thioester intermediate (By

Alternative splicing; ATP-binding; Chromatin regulator; Complete proteome; Disease mutation; DNA damage; DNA repair; Ligase; Mental retardation; Nucleotide-binding; Reference proteome; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0033503; C:HULC complex; IDA:UniProtKB.
GO:0000790; C:nuclear chromatin; IEA:Compara.
GO:0001741; C:XY body; IEA:Compara.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
GO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome.
GO:0033522; P:histone H2A ubiquitination; IDA:UniProtKB.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB.
GO:0006301; P:postreplication repair; NAS:UniProtKB.
GO:0051865; P:protein autoubiquitination; IDA:UniProtKB.
GO:0070979; P:protein K11-linked ubiquitination; IDA:UniProtKB.
GO:0070936; P:protein K48-linked ubiquitination; IDA:UniProtKB.
GO:0009411; P:response to UV; IGI:UniProtKB.
GO:0006511; P:ubiquitin-dependent protein catabolic process; NAS:UniProtKB.

IPR000608; UBQ-conjugat_E2.
IPR023313; UBQ-conjugating_AS.
IPR016135; UBQ-conjugating_enzyme/RWD.

PS00183; UBIQUITIN_CONJUGAT_1
PS50127; UBIQUITIN_CONJUGAT_2