UniProt Accession

 AMD_MOUSE; P97467; E9QL07 

Genbank Protein ID

 U79523; AC102191; AC157923 

Genbank Nucleotide ID

 AAB38364.1 

Protein Name

 Peptidyl-glycine alpha-amidating monooxygenase 

Protein Synonyms/Alias

 PAM; Peptidylglycine alpha-hydroxylating monooxygenase; PHM; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; Peptidylamidoglycolate lyase; PAL 

Gene Name

 Pam 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
922	VLGRLRGKGSSGLNL	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity (By similarity). 

Sequence Annotation

 REPEAT 501 544 NHL 1.
 REPEAT 570 611 NHL 2.
 REPEAT 620 665 NHL 3.
 REPEAT 673 717 NHL 4.
 REPEAT 769 812 NHL 5.
 REGION 1 497 Peptidylglycine alpha-hydroxylating
 REGION 498 823 Peptidyl-alpha-hydroxyglycine alpha-
 REGION 931 948 Interaction with RASSF9 (By similarity).
 METAL 106 106 Copper A (By similarity).
 METAL 107 107 Copper A (By similarity).
 METAL 171 171 Copper A (By similarity).
 METAL 241 241 Copper B (By similarity).
 METAL 243 243 Copper B (By similarity).
 METAL 313 313 Copper B (By similarity).
 MOD_RES 924 924 Phosphoserine.
 MOD_RES 925 925 Phosphoserine.
 MOD_RES 935 935 Phosphoserine (By similarity).
 MOD_RES 948 948 Phosphoserine.
 MOD_RES 949 949 Phosphothreonine (By similarity).
 MOD_RES 952 952 Phosphoserine; by UHMK1.
 CARBOHYD 765 765 N-linked (GlcNAc...) (Potential).
 DISULFID 46 185 By similarity.
 DISULFID 80 125 By similarity.
 DISULFID 113 130 By similarity.
 DISULFID 226 333 By similarity.
 DISULFID 292 314 By similarity.
 DISULFID 634 655 By similarity.
 DISULFID 702 713 By similarity.  

Keyword

 Cleavage on pair of basic residues; Complete proteome; Copper; Cytoplasmic vesicle; Disulfide bond; Glycoprotein; Lyase; Membrane; Metal-binding; Monooxygenase; Multifunctional enzyme; Oxidoreductase; Phosphoprotein; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix; Vitamin C; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 979 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:MGI.
 GO:0005887; C:integral to plasma membrane; TAS:MGI.
 GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005507; F:copper ion binding; IEA:InterPro.
 GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
 GO:0004598; F:peptidylamidoglycolate lyase activity; IEA:EC.
 GO:0004504; F:peptidylglycine monooxygenase activity; TAS:MGI.
 GO:0006518; P:peptide metabolic process; TAS:MGI. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR014784; Cu2_ascorb_mOase-like_C.
 IPR020611; Cu2_ascorb_mOase_CS-1.
 IPR014783; Cu2_ascorb_mOase_CS-2.
 IPR000323; Cu2_ascorb_mOase_N.
 IPR001258; NHL_repeat.
 IPR013017; NHL_repeat_subgr.
 IPR000720; Pep_amidat_mOase.
 IPR008977; PHM/PNGase_F_dom. 

Pfam

 PF01082; Cu2_monooxygen
 PF01436; NHL 

SMART

  

PROSITE

 PS00084; CU2_MONOOXYGENASE_1
 PS00085; CU2_MONOOXYGENASE_2
 PS51125; NHL 

PRINTS

 PR00790; PAMONOXGNASE.