CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006968
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor GTPase-activating protein GLO3 
Protein Synonyms/Alias
 ARF GAP GLO3 
Gene Name
 GLO3 
Gene Synonyms/Alias
 YER122C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
199NTGSLAPKNNTTGSTubiquitination[1]
273KKEAQQEKEDEFTNSubiquitination[1]
430SSYFGEDKEVDEFGNubiquitination[2]
482VVEQGAEKLGSYLRDubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
 GTPase-activating protein for the ADP ribosylation factor (ARF) family. Involved in retrograde vesicular transport from the Golgi to the endoplasmic reticulum. 
Sequence Annotation
 DOMAIN 16 137 Arf-GAP.
 ZN_FING 31 54 C4-type.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 389 389 Phosphoserine.
 MOD_RES 398 398 Phosphoserine.  
Keyword
 Complete proteome; Golgi apparatus; GTPase activation; Metal-binding; Phosphoprotein; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 493 AA 
Protein Sequence
MSNDEGETFA TEQTTQQVFQ KLGSNMENRV CFDCGNKNPT WTSVPFGVML CIQCSAVHRN 60
MGVHITFVKS STLDKWTINN LRRFKLGGNH KARDFFLKNN GKQLLNTANV DAKTKYTSPV 120
AKKYKIHLDK KVQKDMELYP SELVLNGQDS SDSPLDTDSD ASRSTSKENS VDDFFSNWQK 180
PSSNSSSKLN VNTGSLAPKN NTTGSTPKTT VTKTRSSILT ASRKKPVLNS QDKKKHSILS 240
SSRKPTRLTA KKVDKSQAED LFDQFKKEAQ QEKEDEFTNS SSSTKIRQND YDSQFMNNSK 300
GNNNNSIDDI NTQPDEFNDF LNDTSNSFDT TRKEQQDTLT PKFAKLGFGM TMNDANDLAK 360
QQKESQKIAQ GPRYTGRIAE RYGTQKAISS DQLFGRGSFD EAANREAHDK LKTFDNATSI 420
SSSSYFGEDK EVDEFGNPIN SSGSGAGNFD GRNSNNGFID FNASADDELQ MLRDVVEQGA 480
EKLGSYLRDY LRK 493 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IPI:SGD.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IPI:SGD.
 GO:0008060; F:ARF GTPase activator activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0048205; P:COPI coating of Golgi vesicle; IMP:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IDA:SGD. 
Interpro
 IPR001164; ArfGAP. 
Pfam
 PF01412; ArfGap 
SMART
 SM00105; ArfGap 
PROSITE
 PS50115; ARFGAP 
PRINTS
 PR00405; REVINTRACTNG.