CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018898
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor IIIB 90 kDa subunit 
Protein Synonyms/Alias
 TFIIIB90; hTFIIIB90; B-related factor 1; BRF-1; hBRF; TAF3B2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2 
Gene Name
 BRF1 
Gene Synonyms/Alias
 BRF; GTF3B; TAF3B2; TAF3C 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
79GFHVNLGKESRAQTLubiquitination[1]
199HLLEFGEKNHEVSMTubiquitination[1]
247HDFRRTVKEVISVVKubiquitination[1]
254KEVISVVKVCESTLRubiquitination[1]
304GQRKLRMKQLEQVLSubiquitination[1]
340ENSRPKAKGGLASLAubiquitination[1]
537EQKKISSKINYSVLRubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. 
Sequence Annotation
 REPEAT 91 172 1.
 REPEAT 185 269 2.
 ZN_FING 2 33 TFIIB-type.
 METAL 6 6 Zinc (By similarity).
 METAL 9 9 Zinc (By similarity).
 METAL 25 25 Zinc (By similarity).
 METAL 28 28 Zinc (By similarity).
 MOD_RES 365 365 Phosphothreonine.
 MOD_RES 433 433 Phosphoserine (By similarity).
 MOD_RES 450 450 Phosphoserine.
 MOD_RES 553 553 Phosphoserine.  
Keyword
 Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 60
DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 120
HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA 180
IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH 240
DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK 300
LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC 360
GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS 420
LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE 480
YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY 540
SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP 600
AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ 660
MMGSNDYGCD GDEDDGY 677 
Gene Ontology
 GO:0000126; C:transcription factor TFIIIB complex; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0043488; P:regulation of mRNA stability; IEA:Compara.
 GO:0009303; P:rRNA transcription; TAS:ProtInc.
 GO:0006384; P:transcription initiation from RNA polymerase III promoter; TAS:ProtInc.
 GO:0009304; P:tRNA transcription; TAS:ProtInc. 
Interpro
 IPR011665; BRF1_TBP-bd.
 IPR013763; Cyclin-like.
 IPR000812; TFIIB.
 IPR013150; TFIIB_cyclin.
 IPR013137; Znf_TFIIB. 
Pfam
 PF07741; BRF1
 PF08271; TF_Zn_Ribbon
 PF00382; TFIIB 
SMART
 SM00385; CYCLIN 
PROSITE
 PS00782; TFIIB
 PS51134; ZF_TFIIB 
PRINTS
 PR00685; TIFACTORIIB.