UniProt Accession

 HXK2_HUMAN; P52789; D6W5J2; Q8WU87; Q9UN82 

Genbank Protein ID

 Z46376; Z46354; Z46355; Z46604; Z46356; Z46357; Z46358; Z46359; Z46360; Z46361; Z46362; Z46363; Z46364; Z46365; Z46366; Z46367; Z46368; Z46369; AF148513; AY623118; CH471053; CH471053; BC021116; BC064369; D25412 

Genbank Nucleotide ID

 CAA86511.1; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; CAA86476.2; AAD30174.1; AAT38114.1; EAW99601.1; EAW99602.1; AAH21116.1; AAH64369.1; BAA04999.1 

Protein Name

 Hexokinase-2 

Protein Synonyms/Alias

 Hexokinase type II; HK II; Muscle form hexokinase 

Gene Name

 HK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
41	ETLLEISKRFRKEME	ubiquitination	[1, 2]
49	RFRKEMEKGLGATTH	ubiquitination	[2]
62	THPTAAVKMLPTFVR	ubiquitination	[2]
104	VTDNGLQKVEMENQI	ubiquitination	[1, 2, 3]
147	KLQIKDKKLPLGFTF	ubiquitination	[2]
173	SFLVSWTKGFKSSGV	ubiquitination	[1, 2, 3]
176	VSWTKGFKSSGVEGR	ubiquitination	[2, 3]
290	MGSLNPGKQLFEKMI	ubiquitination	[1, 3]
323	EELLFGGKLSPELLN	ubiquitination	[1, 2, 3]
337	NTGRFETKDISDIEG	ubiquitination	[1, 2, 3]
346	ISDIEGEKDGIRKAR	ubiquitination	[2]
418	GVDGSVYKKHPHFAK	ubiquitination	[1, 2]
419	VDGSVYKKHPHFAKR	ubiquitination	[2]
451	RSEDGSGKGAAMVTA	ubiquitination	[1, 2]
472	DQHRARQKTLEHLQL	ubiquitination	[2]
488	HDQLLEVKRRMKVEM	ubiquitination	[1, 2, 3]
492	LEVKRRMKVEMERGL	ubiquitination	[2]
501	EMERGLSKETHASAP	ubiquitination	[2]
624	LKWTKGFKASGCEGE	ubiquitination	[2]
638	EDVVTLLKEAIHRRE	ubiquitination	[2]
738	ELSLNPGKQRFEKMI	ubiquitination	[1]
743	PGKQRFEKMISGMYL	ubiquitination	[2]
763	NILIDFTKRGLLFRG	ubiquitination	[1, 2, 3]
866	GVDGTLYKLHPHFAK	ubiquitination	[1, 2]
885	TVKDLAPKCDVSFLQ	ubiquitination	[2]
899	QSEDGSGKGAALITA	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

  

Sequence Annotation

 NP_BIND 84 89 ATP 1 (Potential).
 NP_BIND 425 426 ATP 1 (By similarity).
 NP_BIND 532 537 ATP 2 (By similarity).
 NP_BIND 747 748 ATP 2 (By similarity).
 NP_BIND 784 788 ATP 2 (By similarity).
 NP_BIND 863 867 ATP 2 (By similarity).
 REGION 1 12 Hydrophobic.
 REGION 13 475 Regulatory.
 REGION 84 88 Glucose-6-phosphate 1 binding.
 REGION 155 156 Substrate 1 binding.
 REGION 172 173 Substrate 1 binding.
 REGION 208 209 Substrate 1 binding.
 REGION 291 294 Substrate 1 binding.
 REGION 413 415 Glucose-6-phosphate 1 binding.
 REGION 476 917 Catalytic.
 REGION 532 536 Glucose-6-phosphate 2 binding.
 REGION 603 604 Substrate 2 binding.
 REGION 620 621 Substrate 2 binding.
 REGION 656 657 Substrate 2 binding.
 REGION 682 683 Substrate 2 binding.
 REGION 739 742 Substrate 2 binding.
 REGION 861 863 Glucose-6-phosphate 2 binding.
 BINDING 30 30 ATP 1 (By similarity).
 BINDING 209 209 Glucose-6-phosphate 1.
 BINDING 232 232 Glucose-6-phosphate 1.
 BINDING 235 235 Substrate 1.
 BINDING 260 260 Substrate 1.
 BINDING 449 449 Glucose-6-phosphate 1.
 BINDING 558 558 ATP (Potential).
 BINDING 657 657 Glucose-6-phosphate 2.
 BINDING 680 680 ATP 2 (By similarity).
 BINDING 680 680 Glucose-6-phosphate 2.
 BINDING 708 708 Substrate 2.
 BINDING 897 897 Glucose-6-phosphate 2.
 MOD_RES 461 461 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Allosteric enzyme; ATP-binding; Complete proteome; Glycolysis; Kinase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 917 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005741; C:mitochondrial outer membrane; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008865; F:fructokinase activity; IBA:RefGenome.
 GO:0004340; F:glucokinase activity; IBA:RefGenome.
 GO:0005536; F:glucose binding; IEA:Compara.
 GO:0019158; F:mannokinase activity; IBA:RefGenome.
 GO:0008637; P:apoptotic mitochondrial changes; IDA:MGI.
 GO:0001678; P:cellular glucose homeostasis; IBA:RefGenome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0006096; P:glycolysis; IBA:RefGenome.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0046324; P:regulation of glucose import; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome. 

Interpro

 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 

Pfam

 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 

SMART

  

PROSITE

 PS00378; HEXOKINASES 

PRINTS

 PR00475; HEXOKINASE.