UniProt Accession

 PHB_MOUSE; P67778; P24142; Q3UB75; Q5SQG4 

Genbank Protein ID

 X78682; AK002714; AK010619; AK150956; AK151073; AK168248; AK168656; AL732490; BC083354; BC089034 

Genbank Nucleotide ID

 CAA55349.1; BAB22305.1; BAB27067.1; BAE29988.1; BAE30089.1; BAE40198.1; BAE40512.1; CAI24279.1; AAH83354.1; AAH89034.1 

Protein Name

 Prohibitin 

Protein Synonyms/Alias

 B-cell receptor-associated protein 32; BAP 32 

Gene Name

 Phb 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
4	****MAAKVFESIGK	acetylation	[1]
128	SITTEILKSVVARFD	acetylation	[1, 2, 3, 4]
128	SITTEILKSVVARFD	ubiquitination	[5]
186	FTEAVEAKQVAQQEA	acetylation	[1, 2, 3, 4]
186	FTEAVEAKQVAQQEA	ubiquitination	[5]
202	RARFVVEKAEQQKKA	acetylation	[1, 3, 4, 6, 7, 8]
202	RARFVVEKAEQQKKA	succinylation	[8]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [8] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging (By similarity). 

Sequence Annotation

 MOD_RES 202 202 N6-acetyllysine (By similarity).
 MOD_RES 249 249 Phosphotyrosine.  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; DNA synthesis; Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 272 AA 

Protein Sequence

Gene Ontology

 GO:0005887; C:integral to plasma membrane; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; IDA:MGI.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0071354; P:cellular response to interleukin-6; IEA:Compara.
 GO:0071897; P:DNA biosynthetic process; IMP:MGI.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0016575; P:histone deacetylation; IEA:Compara.
 GO:0060766; P:negative regulation of androgen receptor signaling pathway; IEA:Compara.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:2000323; P:negative regulation of glucocorticoid receptor signaling pathway; IEA:Compara.
 GO:0010944; P:negative regulation of transcription by competitive promoter binding; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0050847; P:progesterone receptor signaling pathway; IEA:Compara. 

Interpro

 IPR001107; Band_7.
 IPR000163; Prohibitin. 

Pfam

 PF01145; Band_7 

SMART

 SM00244; PHB 

PROSITE

  

PRINTS

 PR00679; PROHIBITIN.