CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructokinase, liver type 
Protein Synonyms/Alias
 Phosphofructo-1-kinase isozyme B; PFK-B; Phosphofructokinase 1; Phosphohexokinase 
Gene Name
 Pfkl 
Gene Synonyms/Alias
 Pfk-l; Pfkb 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
8MATVDLEKLRMSGAGubiquitination[1]
629GLVLRNEKCHEHYTTacetylation[2]
677FDRNYGTKLGVKAMLacetylation[3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP. 
Sequence Annotation
 NP_BIND 35 39 ATP (By similarity).
 NP_BIND 193 197 ATP (By similarity).
 NP_BIND 210 226 ATP (By similarity).
 ACT_SITE 166 166 Proton acceptor (By similarity).
 METAL 224 224 Magnesium; via carbonyl oxygen (By
 BINDING 201 201 Substrate (By similarity).
 BINDING 292 292 Substrate (By similarity).
 BINDING 298 298 Substrate (By similarity).
 BINDING 301 301 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 640 640 Phosphotyrosine.
 MOD_RES 775 775 Phosphoserine.
 CARBOHYD 529 529 O-linked (GlcNAc...) (By similarity).  
Keyword
 Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Glycoprotein; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 780 AA 
Protein Sequence
MATVDLEKLR MSGAGKAIGV LTSGGDAQGM NAAVRAVTRM GIYVGAKVFL IYEGYEGLVE 60
GGENIKPANW LSVSNIIQLG GTIIGSARCK AFTTREGRLA AAYNLLQHGI TNLCVIGGDG 120
SLTGANIFRN EWGSLLEELV KEGKISESTA QNYAHLTIAG LVGSIDNDFC GTDMTIGTDS 180
ALHRIMEVID AITTTAQSHQ RTFVLEVMGR HCGYLALVSA LASGADWLFI PEAPPEDGWE 240
NFMCERLGET RSRGSRLNII IIAEGAIDRH GKPISSSYVK DLVVQRLGFD TRVTVLGHVQ 300
RGGTPSAFDR ILSSKMGMEA VMALLEATPD TPACVVSLSG NQSVRLPLME CVQVTKDVQK 360
AMDEERFDEA IQLRGRSFEN NWKIYKLLAH QKVSKEKSNF SLAILNVGAP AAGMNAAVRS 420
AVRTGISEGH TVYIVHDGFE GLAKGQVQEV GWHDVAGWLG RGGSMLGTKR TLPKPHLEAI 480
VENLRTYNIH ALLVIGGFEA YEGVLQLVEA RGRYEELCIV MCVIPATISN NVPGTDFSLG 540
SDTAVNAAME SCDRIKQSAS GTKRRVFIVE TMGGYCGYLA TVTGIAVGAD AAYVFEDPFN 600
IHDLKANVEH MTEKMKTDIQ RGLVLRNEKC HEHYTTEFLY NLYSSEGRGV FDCRTNVLGH 660
LQQGGAPTPF DRNYGTKLGV KAMLWVSEKL RDVYRKGRVF ANAPDSACVI GLRKKVVAFS 720
PVTELKKETD FEHRMPREQW WLNLRLMLKM LAHYRISMAD YVSGELEHVT RRTLSIDKGF 780 
Gene Ontology
 GO:0005945; C:6-phosphofructokinase complex; IEA:Compara.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0003872; F:6-phosphofructokinase activity; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0070061; F:fructose binding; IEA:Compara.
 GO:0070095; F:fructose-6-phosphate binding; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030388; P:fructose 1,6-bisphosphate metabolic process; ISS:UniProtKB.
 GO:0006002; P:fructose 6-phosphate metabolic process; IDA:MGI.
 GO:0006096; P:glycolysis; IDA:MGI.
 GO:0046676; P:negative regulation of insulin secretion; IDA:MGI.
 GO:0051289; P:protein homotetramerization; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IDA:MGI. 
Interpro
 IPR009161; 6-phosphofructokinase_euk.
 IPR022953; Phosphofructokinase.
 IPR015912; Phosphofructokinase_CS.
 IPR000023; Phosphofructokinase_dom. 
Pfam
 PF00365; PFK 
SMART
  
PROSITE
 PS00433; PHOSPHOFRUCTOKINASE 
PRINTS
 PR00476; PHFRCTKINASE.