CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012395
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIF22 
Protein Synonyms/Alias
 Kinesin-like DNA-binding protein; Kinesin-like protein 4 
Gene Name
 KIF22 
Gene Synonyms/Alias
 KID; KNSL4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32AGRCRLSKIGATRRPubiquitination[1]
87RNHQETLKYQFDAFYubiquitination[1]
133YGPTGAGKTHTMLGSubiquitination[1]
213LIPGLSQKPISSFADubiquitination[1, 2, 3]
269PFRQREGKLYLIDLAubiquitination[1]
292GNKGLRLKESGAINTubiquitination[1]
324RVPYRDSKLTRLLQDubiquitination[1]
366LNFAARSKEVINRPFubiquitination[1]
391GPVKLSQKELLGPPEubiquitination[1, 2, 3]
400LLGPPEAKRARGPEEubiquitination[1]
425APASASQKLSPLQKLubiquitination[1, 2]
431QKLSPLQKLSSMDPAubiquitination[1]
465APLLSTPKRERMVLMacetylation[4]
465APLLSTPKRERMVLMubiquitination[1]
552NAEIHILKNKGRKRKubiquitination[1, 2]
554EIHILKNKGRKRKLEubiquitination[1]
591LLAHGRQKILDLLNEubiquitination[1, 5, 6]
643RVEGITGKQMESFLKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA. 
Sequence Annotation
 DOMAIN 40 299 Kinesin-motor.
 NP_BIND 127 134 ATP (By similarity).
 MOD_RES 412 412 Phosphoserine.
 MOD_RES 427 427 Phosphoserine.
 MOD_RES 452 452 Phosphoserine.
 MOD_RES 543 543 Phosphoserine.
 MOD_RES 562 562 Phosphoserine.
 MOD_RES 581 581 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; DNA-binding; Dwarfism; Microtubule; Motor protein; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 665 AA 
Protein Sequence
MAAGGSTQQR RREMAAASAA AISGAGRCRL SKIGATRRPP PARVRVAVRL RPFVDGTAGA 60
SDPPCVRGMD SCSLEIANWR NHQETLKYQF DAFYGERSTQ QDIYAGSVQP ILRHLLEGQN 120
ASVLAYGPTG AGKTHTMLGS PEQPGVIPRA LMDLLQLTRE EGAEGRPWAL SVTMSYLEIY 180
QEKVLDLLDP ASGDLVIRED CRGNILIPGL SQKPISSFAD FERHFLPASR NRTVGATRLN 240
QRSSRSHAVL LVKVDQRERL APFRQREGKL YLIDLAGSED NRRTGNKGLR LKESGAINTS 300
LFVLGKVVDA LNQGLPRVPY RDSKLTRLLQ DSLGGSAHSI LIANIAPERR FYLDTVSALN 360
FAARSKEVIN RPFTNESLQP HALGPVKLSQ KELLGPPEAK RARGPEEEEI GSPEPMAAPA 420
SASQKLSPLQ KLSSMDPAML ERLLSLDRLL ASQGSQGAPL LSTPKRERMV LMKTVEEKDL 480
EIERLKTKQK ELEAKMLAQK AEEKENHCPT MLRPLSHRTV TGAKPLKKAV VMPLQLIQEQ 540
AASPNAEIHI LKNKGRKRKL ESLDALEPEE KAEDCWELQI SPELLAHGRQ KILDLLNEGS 600
ARDLRSLQRI GPKKAQLIVG WRELHGPFSQ VEDLERVEGI TGKQMESFLK ANILGLAAGQ 660
RCGAS 665 
Gene Ontology
 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0000776; C:kinetochore; TAS:ProtInc.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005819; C:spindle; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003777; F:microtubule motor activity; TAS:ProtInc.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0006281; P:DNA repair; IEA:InterPro.
 GO:0007018; P:microtubule-based movement; TAS:Reactome.
 GO:0007067; P:mitosis; TAS:ProtInc. 
Interpro
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR026986; KIF22.
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00278; HhH1
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.