CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005435
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General negative regulator of transcription subunit 1 
Protein Synonyms/Alias
 Cell division cycle protein 39 
Gene Name
 CDC39 
Gene Synonyms/Alias
 NOT1; ROS1; YCR093W; YCR1151; YCR93W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
722AEYPQYCKDLLRDVPubiquitination[1]
752ATLANAPKERSRPVQubiquitination[1]
783INQEGAPKDVVEKVLubiquitination[1]
1329QQQQQLQKSRLNQPSubiquitination[1]
1384QIKENATKNNLAELGubiquitination[1]
1550ENVKKFIKEFEDTKIubiquitination[1]
1891IDLHSLKKPVDNYLRubiquitination[1]
1921KDTYDIKKGVGYDFLubiquitination[1]
2084QLVKYSKKYTTSEQDubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC- type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. 
Sequence Annotation
 MOD_RES 2102 2102 Phosphothreonine.  
Keyword
 3D-structure; Activator; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2108 AA 
Protein Sequence
MLSATYRDLN TASNLETSKE KQAAQIVIAQ ISLLFTTLNN DNFESVEREI RHILDRSSVD 60
IYIKVWERLL TLSSRDILQA GKFLLQENLL HRLLLEFAKD LPKKSTDLIE LLKERTFNNQ 120
EFQKQTGITL SLFIDLFDKS ANKDIIESLD RSSQINDFKT IKMNHTNYLR NFFLQTTPET 180
LESNLRDLLH SLEGESLNDL LALLLSEILS PGSQNLQNDP TRSWLTPPMV LDATNRGNVI 240
ARSISSLQAN QINWNRVFNL MSTKYFLSAP LMPTTASLSC LFAALHDGPV IDEFFSCDWK 300
VIFKLDLAIQ LHKWSVQNGC FDLLNAEGTR KVSETIPNTK QSLLYLLSIA SLNLELFLQR 360
EELSDGPMLA YFQECFFEDF NYAPEYLILA LVKEMKRFVL LIENRTVIDE ILITLLIQVH 420
NKSPSSFKDV ISTITDDSKI VDAAKIIINS DDAPIANFLK SLLDTGRLDT VINKLPFNEA 480
FKILPCARQI GWEGFDTFLK TKVSPSNVDV VLESLEVQTK MTDTNTPFRS LKTFDLFAFH 540
SLIEVLNKCP LDVLQLQRFE SLEFSLLIAF PRLINFGFGH DEAILANGDI AGINNDIEKE 600
MQNYLQKMYS GELAIKDVIE LLRRLRDSDL PRDQEVFTCI THAVIAESTF FQDYPLDALA 660
TTSVLFGSMI LFQLLRGFVL DVAFRIIMRF AKEPPESKMF KFAVQAIYAF RIRLAEYPQY 720
CKDLLRDVPA LKSQAQVYQS IVEAATLANA PKERSRPVQE MIPLKFFAVD EVSCQINQEG 780
APKDVVEKVL FVLNNVTLAN LNNKVDELKK SLTPNYFSWF STYLVTQRAK TEPNYHDLYS 840
KVIVAMGSGL LHQFMVNVTL RQLFVLLSTK DEQAIDKKHL KNLASWLGCI TLALNKPIKH 900
KNIAFREMLI EAYKENRLEI VVPFVTKILQ RASESKIFKP PNPWTVGILK LLIELNEKAN 960
WKLSLTFEVE VLLKSFNLTT KSLKPSNFIN TPEVIETLSG ALGSITLEQQ QTEQQRQIIL 1020
MQQHQQQMLI YQQRQQQQQQ RQQQQQHHIS ANTIADQQAA FGGEGSISHD NPFNNLLGST 1080
IFVTHPDLKR VFQMALAKSV REILLEVVEK SSGIAVVTTT KIILKDFATE VDESKLKTAA 1140
IIMVRHLAQS LARATSIEPL KEGIRSTMQS LAPNLMSLSS SPAEELDTAI NENIGIALVL 1200
IEKASMDKST QDLADQLMQA IAIRRYHKER RADQPFITQN TNPYSLSLPE PLGLKNTGVT 1260
PQQFRVYEEF GKNIPNLDVI PFAGLPAHAP PMTQNVGLTQ PQQQQAQMPT QILTSEQIRA 1320
QQQQQQLQKS RLNQPSQSAQ PPGVNVPNPQ GGIAAVQSDL EQNQRVLVHL MDILVSQIKE 1380
NATKNNLAEL GDQNQIKTII FQILTFIAKS AQKDQLALKV SQAVVNSLFA TSESPLCREV 1440
LSLLLEKLCS LSLVARKDVV WWLVYALDSR KFNVPVIRSL LEVNLIDATE LDNVLVTAMK 1500
NKMENSTEFA MKLIQNTVLS DDPILMRMDF IKTLEHLASS EDENVKKFIK EFEDTKIMPV 1560
RKGTKTTRTE KLYLVFTEWV KLLQRVENND VITTVFIKQL VEKGVISDTD NLLTFVKSSL 1620
ELSVSSFKES DPTDEVFIAI DALGSLIIKL LILQGFKDDT RRDYINAIFS VIVLVFAKDH 1680
SQEGTTFNER PYFRLFSNIL YEWATIRTHN FVRISDSSTR QELIEFDSVF YNTFSGYLHA 1740
LQPFAFPGFS FAWVTLLSHR MLLPIMLRLP NKIGWEKLML LIIDLFKFLD QYTSKHAVSD 1800
AVSVVYKGTL RVILGISNDM PSFLIENHYE LMNNLPPTYF QLKNVILSAI PKNMTVPNPY 1860
DVDLNMEDIP ACKELPEVFF DPVIDLHSLK KPVDNYLRIP SNSLLRTILS AIYKDTYDIK 1920
KGVGYDFLSV DSKLIRAIVL HVGIEAGIEY KRTSSNAVFN TKSSYYTLLF NLIQNGSIEM 1980
KYQIILSIVE QLRYPNIHTY WFSFVLMNMF KSDEWNDQKL EVQEIILRNF LKRIIVNKPH 2040
TWGVSVFFTQ LINNNDINLL DLPFVQSVPE IKLILQQLVK YSKKYTTSEQ DDQSATINRR 2100
QTPLQSNA 2108 
Gene Ontology
 GO:0030015; C:CCR4-NOT core complex; IPI:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD.
 GO:0051726; P:regulation of cell cycle; IMP:SGD.
 GO:0000749; P:response to pheromone involved in conjugation with cellular fusion; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD. 
Interpro
 IPR007196; CCR4-Not_Not1_C.
 IPR024557; CCR4-Not_Not1su_DUF3819. 
Pfam
 PF12842; DUF3819
 PF04054; Not1 
SMART
  
PROSITE
  
PRINTS