CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014310
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional repressor CTCF 
Protein Synonyms/Alias
 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog 
Gene Name
 Ctcf 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
74DPTLLQMKTEVMEGTsumoylation[1]
405MRTHSGEKPYECYICacetylation[2]
496HKNEKRFKCDQCDYAacetylation[2]
698ENIIVEVKKEPDAEPsumoylation[1]
Reference
 [1] The CTCF insulator protein is posttranslationally modified by SUMO.
 MacPherson MJ, Beatty LG, Zhou W, Du M, Sadowski PD.
 Mol Cell Biol. 2009 Feb;29(3):714-25. [PMID: 19029252]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. 
Sequence Annotation
 ZN_FING 266 288 C2H2-type 1.
 ZN_FING 294 316 C2H2-type 2.
 ZN_FING 322 345 C2H2-type 3.
 ZN_FING 351 373 C2H2-type 4.
 ZN_FING 379 401 C2H2-type 5.
 ZN_FING 407 430 C2H2-type 6.
 ZN_FING 437 460 C2H2-type 7.
 ZN_FING 467 489 C2H2-type 8.
 ZN_FING 495 517 C2H2-type 9.
 ZN_FING 523 546 C2H2-type 10.
 ZN_FING 555 577 C2H2-type 11; atypical.
 MOD_RES 289 289 Phosphothreonine (By similarity).
 MOD_RES 317 317 Phosphothreonine (By similarity).
 MOD_RES 374 374 Phosphothreonine (By similarity).
 MOD_RES 402 402 Phosphoserine (By similarity).
 MOD_RES 609 609 Phosphoserine (By similarity).
 MOD_RES 610 610 Phosphoserine (By similarity).
 MOD_RES 612 612 Phosphoserine (By similarity).
 CROSSLNK 74 74 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 698 698 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Centromere; Chromatin regulator; Chromosome; Chromosome partition; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 736 AA 
Protein Sequence
MEGEAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM 60
VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV 120
PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ 180
GELPPQEDSS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV 240
NAEKVVGNMK PPKPTKIKKK GVKKTFQCEL CSYTCPRRSN LDRHMKSHTD ERPHKCHLCG 300
RAFRTVTLLR NHLNTHTGTR PHKCPDCDMA FVTSGELVRH RRYKHTHEKP FKCSMCDYAS 360
VEVSKLKRHI RSHTGERPFQ CSLCSYASRD TYKLKRHMRT HSGEKPYECY ICHARFTQSG 420
TMKMHILQKH TENVAKFHCP HCDTVIARKS DLGVHLRKQH SYIEQGKKCR YCDAVFHERY 480
ALIQHQKSHK NEKRFKCDQC DYACRQERHM IMHKRTHTGE KPYACSHCDK TFRQKQLLDM 540
HFKRYHDPNF VPAAFVCSKC GKTFTRRNTM ARHADNCAGP DGVEGENGGE TKKSKRGRKR 600
KMRSKKEDSS DSEENAEPDL DDNEEEEEPA VEIEPEPEPQ PQPPPPPQPV APAPPPAKKR 660
RGRPPGRTNQ PKQNQPTAII QVEDQNTGAI ENIIVEVKKE PDAEPAEGEE EEAQAATTDA 720
PNGDLTPEMI LSMMDR 736 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
 GO:0000793; C:condensed chromosome; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0043035; F:chromatin insulator sequence binding; IEA:Compara.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0009048; P:dosage compensation by inactivation of X chromosome; TAS:MGI.
 GO:0010216; P:maintenance of DNA methylation; IMP:MGI.
 GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0016584; P:nucleosome positioning; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IDA:MGI.
 GO:0035065; P:regulation of histone acetylation; IMP:UniProtKB.
 GO:0031060; P:regulation of histone methylation; IMP:UniProtKB.
 GO:0040030; P:regulation of molecular function, epigenetic; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IDA:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS