CPLM-002533

Genbank Nucleotide ID

Adenine phosphoribosyltransferase

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
34	RDISPVLKDPASFRA	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
114	KAELEIQKDALEPGQ	acetylation	[9]
114	KAELEIQKDALEPGQ	ubiquitination	[2, 6, 7]
167	TSLKGREKLAPVPFF	ubiquitination	[2, 6, 7]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[9] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]

Functional Description

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

MOD_RES 2 2 N-acetylalanine.
MOD_RES 4 4 Phosphoserine.
MOD_RES 15 15 Phosphoserine.
MOD_RES 60 60 Phosphotyrosine.
MOD_RES 66 66 Phosphoserine.
MOD_RES 114 114 N6-acetyllysine.
MOD_RES 135 135 Phosphothreonine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Glycosyltransferase; Phosphoprotein; Polymorphism; Purine salvage; Reference proteome; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005634; C:nucleus; IDA:HPA.
GO:0002055; F:adenine binding; IEA:Compara.
GO:0003999; F:adenine phosphoribosyltransferase activity; TAS:Reactome.
GO:0016208; F:AMP binding; IDA:MGI.
GO:0006168; P:adenine salvage; IEA:Compara.
GO:0044209; P:AMP salvage; IEA:UniProtKB-UniPathway.
GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
GO:0007625; P:grooming behavior; IEA:Compara.
GO:0007595; P:lactation; IEA:Compara.
GO:0006144; P:purine nucleobase metabolic process; TAS:Reactome.
GO:0006166; P:purine ribonucleoside salvage; IEA:UniProtKB-KW.
GO:0043101; P:purine-containing compound salvage; TAS:Reactome.

IPR005764; Ade_phspho_trans.
IPR000836; PRibTrfase_dom.

PF00156; Pribosyltran

PS00103; PUR_PYR_PR_TRANSFER