CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011354
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase ISR1 
Protein Synonyms/Alias
 Inhibition of staurosporine resistance protein 1 
Gene Name
 ISR1 
Gene Synonyms/Alias
 YPR106W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
26PSISNNSKGFAYRQPubiquitination[1]
398LINALLKKDPINLNMubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Probable serine/threonine protein kinase which may function redundantly with MPK1-independent branch of the PCK1 pathway that is presumed to be required for the tolerance to high temperatures and staurosporine. 
Sequence Annotation
 DOMAIN 135 415 Protein kinase.
 NP_BIND 141 149 ATP (By similarity).
 ACT_SITE 280 280 Proton acceptor (By similarity).
 BINDING 169 169 ATP (By similarity).  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 443 AA 
Protein Sequence
MNSTPPTSPV TRVSDGSFPS ISNNSKGFAY RQPQKHKSNF AYSHLVSPVE EPTAKFSEAF 60
QTDYSSKAPV ATSEAHLKND LDVLFTTPRF YSPENLALMF RLSNTVSSLE FLDEFLMGIL 120
LAPEMDFLSN PSYSLPSNKL VGQGSYSYVY PISSSASSRC NNDSGVVLKF AKSQHKSKVI 180
LQEALTLAYL QYMSPSTNES HIIPFYGLTY ITKSHFRRLR SNECVPGLIL PKCEMSLYHF 240
NTAVSHKLSL ITKRKIWWRL MKQMIDALKS LKTNGIIHGD IKTANILITE MHVLNGGHCK 300
DFDFYLADFT SAFHINQTPT DLNTTVEYCA PELIDSSSDH VPTFESDLYA VGLCLLSFIS 360
QHEPYNELQA LVSHGSSPGI GSSSIQQSQW LINALLKKDP INLNMLRNDL FQDWKSELAL 420
LSRILVDRLP LENLITILDS NYI 443 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; ISS:SGD.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS