CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005716
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair protein complementing XP-G cells 
Protein Synonyms/Alias
 DNA excision repair protein ERCC-5; Xeroderma pigmentosum group G-complementing protein 
Gene Name
 ERCC5 
Gene Synonyms/Alias
 ERCM2; XPG; XPGC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MGVQGLWKLLECSGRacetylation[1]
8MGVQGLWKLLECSGRubiquitination[2]
64TLFHRLCKLLFFRIRubiquitination[2, 3, 4]
85GDAPLLKKQTLVKRRubiquitination[2]
111KTTEKLLKTFLKRQAubiquitination[2]
120FLKRQAIKTAFRSKRubiquitination[2]
126IKTAFRSKRDEALPSubiquitination[2]
209HEILTDMKEFTKRRRubiquitination[2, 4]
213TDMKEFTKRRRTLFEubiquitination[2]
235DFSQYQLKGLLKKNYubiquitination[2]
274EDEGGFLKEVESRRVubiquitination[2, 4]
293TSHYILIKGIQAKTVubiquitination[2, 4, 5, 6]
298LIKGIQAKTVAEVDSubiquitination[2, 4]
313ESLPSSSKMHGMSFDubiquitination[2]
322HGMSFDVKSSPCEKLubiquitination[2]
402LDDDEDVKVCAGDDVubiquitination[2, 3]
433ENSDEGLKVRDGKGIubiquitination[2, 4]
830RNFFNKNKFVEYYQYubiquitination[2]
972YFGWNRTKTDESLFPubiquitination[2]
982ESLFPVLKQLDAQQTubiquitination[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too. 
Sequence Annotation
 REGION 1 95 N-domain.
 REGION 753 881 I-domain.
 REGION 981 1009 Interaction with PCNA.
 MOTIF 1057 1073 Nuclear localization signal (Potential).
 METAL 30 30 Magnesium 1 (By similarity).
 METAL 77 77 Magnesium 1 (By similarity).
 METAL 789 789 Magnesium 1 (By similarity).
 METAL 791 791 Magnesium 1 (By similarity).
 METAL 810 810 Magnesium 2 (By similarity).
 METAL 812 812 Magnesium 2 (By similarity).
 METAL 861 861 Magnesium 2 (By similarity).
 MOD_RES 8 8 N6-acetyllysine.
 MOD_RES 705 705 Phosphoserine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Cockayne syndrome; Complete proteome; Deafness; Disease mutation; DNA damage; DNA repair; DNA-binding; Dwarfism; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Xeroderma pigmentosum. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1186 AA 
Protein Sequence
MGVQGLWKLL ECSGRQVSPE ALEGKILAVD ISIWLNQALK GVRDRHGNSI ENPHLLTLFH 60
RLCKLLFFRI RPIFVFDGDA PLLKKQTLVK RRQRKDLASS DSRKTTEKLL KTFLKRQAIK 120
TAFRSKRDEA LPSLTQVRRE NDLYVLPPLQ EEEKHSSEEE DEKEWQERMN QKQALQEEFF 180
HNPQAIDIES EDFSSLPPEV KHEILTDMKE FTKRRRTLFE AMPEESDDFS QYQLKGLLKK 240
NYLNQHIEHV QKEMNQQHSG HIRRQYEDEG GFLKEVESRR VVSEDTSHYI LIKGIQAKTV 300
AEVDSESLPS SSKMHGMSFD VKSSPCEKLK TEKEPDATPP SPRTLLAMQA ALLGSSSEEE 360
LESENRRQAR GRNAPAAVDE GSISPRTLSA IKRALDDDED VKVCAGDDVQ TGGPGAEEMR 420
INSSTENSDE GLKVRDGKGI PFTATLASSS VNSAEEHVAS TNEGREPTDS VPKEQMSLVH 480
VGTEAFPISD ESMIKDRKDR LPLESAVVRH SDAPGLPNGR ELTPASPTCT NSVSKNETHA 540
EVLEQQNELC PYESKFDSSL LSSDDETKCK PNSASEVIGP VSLQETSSIV SVPSEAVDNV 600
ENVVSFNAKE HENFLETIQE QQTTESAGQD LISIPKAVEP MEIDSEESES DGSFIEVQSV 660
ISDEELQAEF PETSKPPSEQ GEEELVGTRE GEAPAESESL LRDNSERDDV DGEPQEAEKD 720
AEDSLHEWQD INLEELETLE SNLLAQQNSL KAQKQQQERI AATVTGQMFL ESQELLRLFG 780
IPYIQAPMEA EAQCAILDLT DQTSGTITDD SDIWLFGARH VYRNFFNKNK FVEYYQYVDF 840
HNQLGLDRNK LINLAYLLGS DYTEGIPTVG CVTAMEILNE FPGHGLEPLL KFSEWWHEAQ 900
KNPKIRPNPH DTKVKKKLRT LQLTPGFPNP AVAEAYLKPV VDDSKGSFLW GKPDLDKIRE 960
FCQRYFGWNR TKTDESLFPV LKQLDAQQTQ LRIDSFFRLA QQEKEDAKRI KSQRLNRAVT 1020
CMLRKEKEAA ASEIEAVSVA MEKEFELLDK AKGKTQKRGI TNTLEESSSL KRKRLSDSKG 1080
KNTCGGFLGE TCLSESSDGS SSEDAESSSL MNVQRRTAAK EPKTSASDSQ NSVKEAPVKN 1140
GGATTSSSSD SDDDGGKEKM VLVTARSVFG KKRRKLRRAR GRKRKT 1186 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000405; F:bubble DNA binding; IDA:UniProtKB.
 GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
 GO:0004520; F:endodeoxyribonuclease activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
 GO:0008340; P:determination of adult lifespan; IEA:Compara.
 GO:0035264; P:multicellular organism growth; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0006295; P:nucleotide-excision repair, DNA incision, 3'-to lesion; IDA:UniProtKB.
 GO:0010225; P:response to UV-C; IMP:UniProtKB.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; IMP:UniProtKB.
 GO:0009650; P:UV protection; IGI:MGI. 
Interpro
 IPR020045; 5-3_exonuclease_C.
 IPR008918; HhH2.
 IPR006086; XPG-I_dom.
 IPR006084; XPG/Rad2.
 IPR001044; XPG/Rad2_eukaryotes.
 IPR019974; XPG_CS.
 IPR006085; XPG_DNA_repair_N. 
Pfam
 PF00867; XPG_I
 PF00752; XPG_N 
SMART
 SM00279; HhH2
 SM00484; XPGI
 SM00485; XPGN 
PROSITE
 PS00841; XPG_1
 PS00842; XPG_2 
PRINTS
 PR00853; XPGRADSUPER.
 PR00066; XRODRMPGMNTG.