UniProt Accession

 MED9_HUMAN; Q9NWA0 

Genbank Protein ID

 AK001055; CH471196; BC104988; BC112029 

Genbank Nucleotide ID

 BAA91483.1; EAW55706.1; AAI04989.1; AAI12030.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 9 

Protein Synonyms/Alias

 Mediator complex subunit 9 

Gene Name

 MED9 

Gene Synonyms/Alias

 MED25 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
99	SKFQEMRKLISTMPG	ubiquitination	[1]
134	TKNELLQKYKSLCMF	ubiquitination	[1, 2, 3]
145	LCMFEIPKE******	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

  

Keyword

 Activator; Coiled coil; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 146 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IEA:Compara.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. 

Interpro

 IPR011425; Mediator_Med9. 

Pfam

 PF07544; Med9 

SMART

  

PROSITE

  

PRINTS