UniProt Accession

 EIF3B_MOUSE; Q8JZQ9; Q922K2 

Genbank Protein ID

 AK033722; AK167618; AK170938; BC007175; BC031704 

Genbank Nucleotide ID

 BAC28445.1; BAE39671.1; BAE42128.1; AAH07175.1; AAH31704.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit B 

Protein Synonyms/Alias

 eIF3b; Eukaryotic translation initiation factor 3 subunit 9; eIF-3-eta; eIF3 p116 

Gene Name

 Eif3b 

Gene Synonyms/Alias

 Eif3s9 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
98	QEPSAPGKAEAVGEQ	ubiquitination	[1]
191	VGPDRLEKLKNVIHK	ubiquitination	[1]
198	KLKNVIHKIFSKFGK	ubiquitination	[1]
202	VIHKIFSKFGKIIND	ubiquitination	[1]
205	KIFSKFGKIINDYYP	acetylation	[2]
205	KIFSKFGKIINDYYP	ubiquitination	[1]
237	AHAVDAVKNADGYKL	ubiquitination	[1]
246	ADGYKLDKQHTFRVN	ubiquitination	[1]
273	DEWDIPEKQPFKDLG	ubiquitination	[1]
277	IPEKQPFKDLGNLRY	ubiquitination	[1]
313	SIFWNDVKDPVSIEE	ubiquitination	[1]
353	IALWGGDKFKQIQRF	acetylation	[2]
353	IALWGGDKFKQIQRF	ubiquitination	[1]
448	PSMGLLDKKSLKISG	ubiquitination	[1]
505	LFNVVDCKLHWQKNG	ubiquitination	[1]
524	VKVDRTPKGTQGVVT	acetylation	[3]
524	VKVDRTPKGTQGVVT	ubiquitination	[1]
541	EIFRMREKQVPVDVV	acetylation	[3]
541	EIFRMREKQVPVDVV	ubiquitination	[1]
593	NGKIELIKMFDKQQA	acetylation	[2]
718	KDLKKYSKIFEQKDR	ubiquitination	[1]
733	LSQSKASKELVERRR	acetylation	[3]
759	MAQELYMKQKNERLE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 DOMAIN 174 257 RRM.
 REPEAT 321 359 WD 1.
 REPEAT 361 406 WD 2.
 REPEAT 410 448 WD 3.
 REPEAT 549 590 WD 4.
 REPEAT 638 683 WD 5.
 REGION 113 402 Sufficient for interaction with EIF3E (By
 REGION 159 263 Sufficient for interaction with EIF3J (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 31 31 Phosphothreonine.
 MOD_RES 68 68 Phosphoserine.
 MOD_RES 74 74 Phosphothreonine.
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 79 79 Phosphoserine.
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 120 120 Phosphoserine.
 MOD_RES 123 123 Phosphoserine.
 MOD_RES 141 141 Phosphoserine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphoserine (By similarity).
 MOD_RES 198 198 N6-acetyllysine (By similarity).
 MOD_RES 228 228 Phosphoserine (By similarity).
 MOD_RES 277 277 N6-acetyllysine (By similarity).
 MOD_RES 353 353 N6-acetyllysine (By similarity).
 MOD_RES 440 440 Phosphothreonine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA-binding; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 803 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR011400; EIF3B.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR013979; TIF_beta_prop-like.
 IPR015943; WD40/YVTN_repeat-like_dom. 

Pfam

 PF08662; eIF2A
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS