CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007111
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Metal resistance protein YCF1 
Protein Synonyms/Alias
 Yeast cadmium factor 1 
Gene Name
 YCF1 
Gene Synonyms/Alias
 YDR135C; YD9302.11C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
504LKLYAWEKPYREKLEacetylation[1]
504LKLYAWEKPYREKLEubiquitination[2]
509WEKPYREKLEEVRNNubiquitination[2]
862YGKKNNGKSNEFGDSubiquitination[2]
890GELEQLQKLNDLDFGubiquitination[2]
1059RFSNDIYKVDALLGRacetylation[1]
1297KHINIHIKPNEKVGIacetylation[1]
1312VGRTGAGKSSLTLALacetylation[1]
1348GLYDLRHKLSIIPQDacetylation[1]
1484IIVLDNGKVAEFDSPubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates. 
Sequence Annotation
 DOMAIN 287 590 ABC transmembrane type-1 1.
 DOMAIN 626 853 ABC transporter 1.
 DOMAIN 951 1235 ABC transmembrane type-1 2.
 DOMAIN 1272 1507 ABC transporter 2.
 NP_BIND 663 670 ATP 1 (Potential).
 NP_BIND 1306 1313 ATP 2 (Potential).
 MOD_RES 251 251 Phosphoserine.
 MOD_RES 873 873 Phosphoserine.
 MOD_RES 903 903 Phosphoserine.
 MOD_RES 908 908 Phosphoserine.
 MOD_RES 911 911 Phosphothreonine.
 MOD_RES 914 914 Phosphoserine.  
Keyword
 ATP-binding; Cadmium resistance; Complete proteome; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1515 AA 
Protein Sequence
MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI RDLVNLCKKK 60
HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN FTIVSQYAST MLSLFVALAL 120
HWIEYDRSVV ANTVLLFYWL FETFGNFAKL INILIRHTYE GIWYSGQTGF ILTLFQVITC 180
ASILLLEALP KKPLMPHQHI HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE 240
ADLYKLPRNF SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL 300
AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF AMFLVGFTQT 360
SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS STGDIVNLMS VDVQKLQDLT 420
QWLNLIWSGP FQIIICLYSL YKLLGNSMWV GVIILVIMMP LNSFLMRIQK KLQKSQMKYK 480
DERTRVISEI LNNIKSLKLY AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP 540
FLVSCCTFAV FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT 600
FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN FQAKKGNLTC 660
IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP WIMNGTVKEN ILFGHRYDAE 720
FYEKTIKACA LTIDLAILMD GDKTLVGEKG ISLSGGQKAR LSLARAVYAR ADTYLLDDPL 780
AAVDEHVARH LIEHVLGPNG LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI 840
TKDADSPLWK LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD 900
AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS VCVFILFIVI 960
SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI GSALATLIQT IVLWVFCTIH 1020
ASKYLHNLMT NSVLRAPMTF FETTPIGRIL NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF 1080
TITVICATTW QFIFIIIPLS VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA 1140
TVRGYSQQKR FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK 1200
QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK SEAPLIVEGH 1260
RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE KVGIVGRTGA GKSSLTLALF 1320
RMIEASEGNI VIDNIAINEI GLYDLRHKLS IIPQDSQVFE GTVRENIDPI NQYTDEAIWR 1380
ALELSHLKEH VLSMSNDGLD AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV 1440
DVETDKVVQE TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS 1500
LFYSLCMEAG LVNEN 1515 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015127; F:bilirubin transmembrane transporter activity; IMP:SGD.
 GO:0015431; F:glutathione S-conjugate-exporting ATPase activity; IDA:SGD.
 GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
 GO:0010038; P:response to metal ion; IDA:SGD.
 GO:0042144; P:vacuole fusion, non-autophagic; IMP:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS