| Tag | Content |
|---|
| CPLM ID | CPLM-004225 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Sensor kinase protein RcsC |
Protein Synonyms/Alias | Capsular synthesis regulator component C |
Gene Name | rcsC |
Gene Synonyms/Alias | b2218; JW5917/JW5920 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 498 | NLDLLQTKELPKGVD | acetylation | [1] | | 530 | SDILDFSKIESEQLK | acetylation | [1] | | 537 | KIESEQLKIEPREFS | acetylation | [1] | | 911 | ANALAEEKQRCLESG | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Member of the two-component regulatory system RcsC/RcsB involved in the regulation of the expression of genes involved in colanic acid capsule synthesis. Together with RcsF, RcsC probably functions as a membrane-associated protein kinase that phosphorylates RcsB in response to environmental signals. The two- component system interacts with a phosphotransfer intermediate, RcsD, which receives a phosphoryl group from RcsC and transfers it to RscB. Activity is probably up-regulated by YmgA/AriR, and possibly down-regulated by YcgZ, all 3 are connector proteins providing additional signal input into the two-component system. |
Sequence Annotation | DOMAIN 357 425 PAS.
DOMAIN 476 692 Histidine kinase.
DOMAIN 705 805 ABL.
DOMAIN 826 940 Response regulatory.
MOD_RES 479 479 Phosphohistidine; by autocatalysis (By
MOD_RES 875 875 4-aspartylphosphate (By similarity). |
Keyword | 3D-structure; ATP-binding; Capsule biogenesis/degradation; Cell inner membrane; Cell membrane; Complete proteome; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Two-component regulatory system. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 949 AA |
Protein Sequence | MKYLASFRTT LKASRYMFRA LALVLWLLIA FSSVFYIVNA LHQRESEIRQ EFNLSSDQAQ 60
RFIQRTSDVM KELKYIAENR LSAENGVLSP RGRETQADVP AFEPLFADSD CSAMSNTWRG 120
SLESLAWFMR YWRDNFSAAY DLNRVFLIGS DNLCMANFGL RDMPVERDTA LKALHERINK 180
YRNAPQDDSG SNLYWISEGP RPGVGYFYAL TPVYLANRLQ ALLGVEQTIR MENFFLPGTL 240
PMGVTILDEN GHTLISLTGP ESKIKGDPRW MQERSWFGYT EGFRELVLKK NLPPSSLSIV 300
YSVPVDKVLE RIRMLILNAI LLNVLAGAAL FTLARMYERR IFIPAESDAL RLEEHEQFNR 360
KIVASAPVGI CILRTADGVN ILSNELAHTY LNMLTHEDRQ RLTQIICGQQ VNFVDVLTSN 420
NTNLQISFVH SRYRNENVAI CVLVDVSSRV KMEESLQEMA QAAEQASQSK SMFLATVSHE 480
LRTPLYGIIG NLDLLQTKEL PKGVDRLVTA MNNSSSLLLK IISDILDFSK IESEQLKIEP 540
REFSPREVMN HITANYLPLV VRKQLGLYCF IEPDVPVALN GDPMRLQQVI SNLLSNAIKF 600
TDTGCIVLHV RADGDYLSIR VRDTGVGIPA KEVVRLFDPF FQVGTGVQRN FQGTGLGLAI 660
CEKLISMMDG DISVDSEPGM GSQFTVRIPL YGAQYPQKKG VEGLSGKRCW LAVRNASLCQ 720
FLETSLQRSG IVVTTYEGQE PTPEDVLITD EVVSKKWQGR AVVTFCRRHI GIPLEKAPGE 780
WVHSVAAPHE LPALLARIYL IEMESDDPAN ALPSTDKAVS DNDDMMILVV DDHPINRRLL 840
ADQLGSLGYQ CKTANDGVDA LNVLSKNHID IVLSDVNMPN MDGYRLTQRI RQLGLTLPVI 900
GVTANALAEE KQRCLESGMD SCLSKPVTLD VIKQTLTLYA ERVRKSRDS 949 |
Gene Ontology | GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro. GO:0000155; F:phosphorelay sensor kinase activity; IDA:EcoCyc. GO:0071470; P:cellular response to osmotic stress; IMP:EcoCyc. GO:0035556; P:intracellular signal transduction; IEA:GOC. GO:0018106; P:peptidyl-histidine phosphorylation; IEA:InterPro. GO:0046777; P:protein autophosphorylation; IMP:EcoCyc. GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. GO:0044010; P:single-species biofilm formation; IMP:EcoCyc. |
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