| Tag | Content |
|---|
| CPLM ID | CPLM-011196 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Myosin-5 |
Protein Synonyms/Alias | Actin-dependent myosin-I MYO5; Class I unconventional myosin MYO5; Type I myosin MYO5 |
Gene Name | MYO5 |
Gene Synonyms/Alias | YMR109W; YM9718.08 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 907 | TELITHLKRLNDKIQ | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | One of two redundant type-I myosins implicated in the organization of the actin cytoskeleton. Required for proper actin cytoskeleton polarization and for the internalization step in endocytosis. At the cell cortex, assembles in patch-like structures together with proteins from the actin-polymerizing machinery and promotes actin assembly. Functions redundantly with LAS17 as actin nucleation-promoting factor (NPF) for the Arp2/3 complex. Motor domain phosphorylation by PAK kinases CLA4 and STE20 promotes CDC42-regulated actin assembly. Functions together with the NPF PAN1 in late stages of endocytosis. Motor domain phosphorylation by PDK1 kinases PKH1 and PKH2, and by SGK kinases YPK1 and YPK2, promotes ligand-induced, but not constitutive endocytosis of the G protein-coupled receptor STE2. |
Sequence Annotation | DOMAIN 38 702 Myosin head-like.
DOMAIN 719 739 IQ 1.
DOMAIN 740 765 IQ 2.
DOMAIN 1085 1147 SH3.
NP_BIND 129 136 ATP (Potential).
REGION 404 486 Actin-binding (By similarity).
REGION 766 961 Basic, putative membrane-binding region.
MOD_RES 357 357 Phosphoserine.
MOD_RES 359 359 Phosphotyrosine.
MOD_RES 777 777 Phosphoserine.
MOD_RES 992 992 Phosphoserine.
MOD_RES 1205 1205 Phosphoserine. |
Keyword | 3D-structure; Actin-binding; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; SH3 domain. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1219 AA |
Protein Sequence | MAILKRGARK KVHQEPAKRS ANIKKATFDS SKKKEVGVSD LTLLSKISDE AINENLKKRF 60
LNATIYTYIG HVLISVNPFR DLGIYTDAVM NEYKGKNRLE VPPHVFAIAE SMYYNMKSYN 120
ENQCVIISGE SGAGKTEAAK RIMQYIAAAS STHTESIGKI KDMVLATNPL LESFGCAKTL 180
RNNNSSRHGK YLEIKFNNQF EPCAGNITNY LLEKQRVVSQ IKNERNFHIF YQFTKGASDA 240
YRQTFGVQKP EQYVYTAAAG CISAETIDDL QDYQETLKAM RVIGLGQEEQ DQIFRMLAAI 300
LWIGNVSFIE NEEGNAQVRD TSVTDFVAYL LQIDSQLLIK SLVERIMETN HGMKRGSVYH 360
VPLNIVQADA VRDALAKAIY NNLFDWIVSR VNKSLQAFPG AEKSIGILDI YGFEIFEHNS 420
FEQICINYVN EKLQQIFIQL TLKSEQETYE REKIQWTPIK YFDNKVVCDL IEARRPPGIF 480
AAMNDSVATA HADSNAADQA FAQRLNLFTT NPHFDLRSNK FVIKHYAGDV TYDIDGITDK 540
NKDQLQKDLV ELIGTTTNTF LATIFPDTVD RESKRRPPTA GDKIIKSAND LVETLSKAQP 600
SYIRTIKPNE TKSPNDYDDR QVLHQIKYLG LQENVRIRRA GFAYRQVFEK FVERFYLLSP 660
HCSYAGDYTW QGDTLDAVKY ILQDSSIPQQ EYQLGVTSVF IKTPETLFAL EHMRDRYWHN 720
MAARIQRAWR RFLQRRIDAA TKIQRTIRER KEGNKYEKLR DYGTKVLGGR KERRSMSLLG 780
YRAFMGDYLS CNESKSKGAY IKRQVSIKEK VIFSIHGEAL HTKFGRSAQR LKKTFLLTPT 840
TLYIVGQTLV QNAMTYTQDY KIDVRNIQAV SLTNLQDDWV AIKLASSGQP DPLINTYFKT 900
ELITHLKRLN DKIQIKIGSA IEYQKKPGKL HSVKCQINES APKYGDIYKS STISVRRGNP 960
PNSQVHKKPR KKSSISSGYH ASSSQATRRP VSIAAAQHVP TAPASRHSKK PAPPPPGMQN 1020
KAATRRSVPN PASTLTASQS NARPSPPTAA TRATPAATPA AAAMGSGRQA NIPPPPPPPP 1080
PSSKPKEPMF EAAYDFPGSG SPSELPLKKG DVIYITREEP SGWSLGKLLD GSKEGWVPTA 1140
YMKPHSGNNN IPTPPQNRDV PKPVLNSVQH DNTSANVIPA AAQASLGDGL ANALAARANK 1200
MRLESDDEEA NEDEEEDDW 1219 |
Gene Ontology | GO:0030479; C:actin cortical patch; IDA:SGD. GO:0043332; C:mating projection tip; IDA:SGD. GO:0016459; C:myosin complex; IEA:UniProtKB-KW. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0000146; F:microfilament motor activity; TAS:SGD. GO:0051666; P:actin cortical patch localization; IMP:SGD. GO:0007121; P:bipolar cellular bud site selection; TAS:SGD. GO:0006887; P:exocytosis; TAS:SGD. GO:0031505; P:fungal-type cell wall organization; TAS:SGD. GO:0006898; P:receptor-mediated endocytosis; IMP:SGD. GO:0009651; P:response to salt stress; IPI:SGD. |
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