UniProt Accession

 YES_RAT; F1LM93 

Genbank Protein ID

 AABR03068393; AABR03068636 

Genbank Nucleotide ID

  

Protein Name

 Tyrosine-protein kinase Yes 

Protein Synonyms/Alias

 p61-Yes 

Gene Name

 Yes1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
233	AQFDTLQKLVKHYTE	acetylation	[1]
431	YTARQGAKFPIKWTA	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity). 

Sequence Annotation

 DOMAIN 89 150 SH3.
 DOMAIN 156 253 SH2.
 DOMAIN 275 528 Protein kinase.
 NP_BIND 281 289 ATP (By similarity).
 ACT_SITE 394 394 Proton acceptor (By similarity).
 BINDING 303 303 ATP (By similarity).
 MOD_RES 11 11 Phosphoserine (By similarity).
 MOD_RES 32 32 Phosphotyrosine (By similarity).
 MOD_RES 109 109 Phosphoserine (By similarity).
 MOD_RES 192 192 Phosphotyrosine (By similarity).
 MOD_RES 193 193 Phosphoserine (By similarity).
 MOD_RES 220 220 Phosphotyrosine (By similarity).
 MOD_RES 221 221 Phosphotyrosine (By similarity).
 MOD_RES 334 334 Phosphotyrosine (By similarity).
 MOD_RES 343 343 Phosphotyrosine (By similarity).
 MOD_RES 424 424 Phosphotyrosine; by autocatalysis (By
 MOD_RES 444 444 Phosphotyrosine (By similarity).
 MOD_RES 535 535 Phosphotyrosine; by CSK (By similarity).
 LIPID 2 2 N-myristoyl glycine (Potential).
 LIPID 3 3 S-palmitoyl cysteine; in membrane form  

Keyword

 ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Lipoprotein; Membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; SH2 domain; SH3 domain; Transferase; Tyrosine-protein kinase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 541 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
 GO:0004713; F:protein tyrosine kinase activity; IDA:RGD.
 GO:0015758; P:glucose transport; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:RGD. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR000980; SH2.
 IPR001452; SH3_domain.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 

Pfam

 PF07714; Pkinase_Tyr
 PF00017; SH2
 PF00018; SH3_1 

SMART

 SM00252; SH2
 SM00326; SH3
 SM00219; TyrKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS50001; SH2
 PS50002; SH3 

PRINTS

 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.
 PR00109; TYRKINASE.