CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022128
UniProt Accession
Genbank Protein ID
 AY750055; AY818645; AB244756; AB244757; AB244758; AL137718; BX649186; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL390878; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL359266; AL390878; AL356502; AL354829; AL359266; AL390878; AL356502; AL354829; AL390878; AL356502; AL354829; AL359266; AL390878; AL356502; AL354829; AL359266; AL390878; AL356502; AL354829; AL359266; AL390878; AL359266; AL354829; AL356502; AL390878; AL359266; AL354829; AL356502; AL390878; AL359266; AL354829; AL356502; AL390878; AL359266; AL354829; AL356502; AL390878; AL390878; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL390878; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL359266; AL390878; AL354829; AL356502; AL359266; BC034952; BC048963; BC068504; AK092024 
Genbank Nucleotide ID
 AAW73254.1; AAW78862.1; BAE96350.1; BAE96351.1; BAE96352.1; CAB70890.1; CAE46204.1; CAI39756.2; CAI39756.2; CAI39756.2; CAI39756.2; CAI39757.2; CAI39757.2; CAI39757.2; CAM14265.1; CAM14265.1; CAM14265.1; CAM14265.1; CAM14266.1; CAM14266.1; CAM14266.1; CAM14266.1; CAM14267.1; CAM14267.1; CAM14267.1; CAM14267.1; CAI14102.2; CAI14102.2; CAI14102.2; CAI14102.2; CAM19398.1; CAM19398.1; CAM19398.1; CAM19399.1; CAM19399.1; CAM19399.1; CAM19399.1; CAM19400.1; CAM19400.1; CAM19400.1; CAM19400.1; CAM19401.1; CAM19401.1; CAM19401.1; CAM19401.1; CAM15403.1; CAM15403.1; CAM15403.1; CAM15403.1; CAM15404.1; CAM15404.1; CAM15404.1; CAM15404.1; CAM15405.1; CAM15405.1; CAM15405.1; CAM15405.1; CAM15406.1; CAM15406.1; CAM15406.1; CAM15406.1; CAI14158.2; CAI14158.2; CAI14158.2; CAI14158.2; CAM19738.1; CAM19738.1; CAM19738.1; CAM19739.1; CAM19739.1; CAM19739.1; CAM19739.1; CAM19740.1; CAM19740.1; CAM19740.1; CAM19740.1; CAM19741.1; CAM19741.1; CAM19741.1; CAM19741.1; AAH34952.1; AAH48963.1; AAH68504.1; BAC03793.1 
Protein Name
 Protein diaphanous homolog 3 
Protein Synonyms/Alias
 Diaphanous-related formin-3; DRF3; MDia2 
Gene Name
 DIAPH3 
Gene Synonyms/Alias
 DIAP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
139DMNLNEDKKAPLREKubiquitination[1, 2]
Reference
 [1] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). 
Sequence Annotation
 DOMAIN 114 476 GBD/FH3.
 DOMAIN 561 631 FH1.
 DOMAIN 636 1034 FH2.
 DOMAIN 1057 1087 DAD.
 MOD_RES 624 624 Phosphoserine.
 CROSSLNK 139 139 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Deafness; Isopeptide bond; Neuropathy; Non-syndromic deafness; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1193 AA 
Protein Sequence
MERHQPRLHH PAQGSAAGTP YPSSASLRGC RESKMPRRKG PQHPPPPSGP EEPGEKRPKF 60
HLNIRTLTDD MLDKFASIRI PGSKKERPPL PNLKTAFASS DCSAAPLEMM ENFPKPLSEN 120
ELLELFEKMM EDMNLNEDKK APLREKDFSI KKEMVMQYIN TASKTGSLKR SRQISPQEFI 180
HELKMGSADE RLVTCLESLR VSLTSNPVSW VESFGHEGLG LLLDILEKLI SGKIQEKVVK 240
KNQHKVIQCL KALMNTQYGL ERIMSEERSL SLLAKAVDPR HPNMMTDVVK LLSAVCIVGE 300
ESILEEVLEA LTSAGEEKKI DRFFCIVEGL RHNSVQLQVA CMQLINALVT SPDDLDFRLH 360
IRNEFMRCGL KEILPNLKCI KNDGLDIQLK VFDEHKEEDL FELSHRLEDI RAELDEAYDV 420
YNMVWSTVKE TRAEGYFISI LQHLLLIRND YFIRQQYFKL IDECVSQIVL HRDGMDPDFT 480
YRKRLDLDLT QFVDICIDQA KLEEFEEKAS ELYKKFEKEF TDHQETQAEL QKKEAKINEL 540
QAELQAFKSQ FGALPADCNI PLPPSKEGGT GHSALPPPPP LPSGGGVPPP PPPPPPPPLP 600
GMRMPFSGPV PPPPPLGFLG GQNSPPLPIL PFGLKPKKEF KPEISMRRLN WLKIRPHEMT 660
ENCFWIKVNE NKYENVDLLC KLENTFCCQQ KERREEEDIE EKKSIKKKIK ELKFLDSKIA 720
QNLSIFLSSF RVPYEEIRMM ILEVDETRLA ESMIQNLIKH LPDQEQLNSL SQFKSEYSNL 780
CEPEQFVVVM SNVKRLRPRL SAILFKLQFE EQVNNIKPDI MAVSTACEEI KKSKSFSKLL 840
ELVLLMGNYM NAGSRNAQTF GFNLSSLCKL KDTKSADQKT TLLHFLVEIC EEKYPDILNF 900
VDDLEPLDKA SKVSVETLEK NLRQMGRQLQ QLEKELETFP PPEDLHDKFV TKMSRFVISA 960
KEQYETLSKL HENMEKLYQS IIGYYAIDVK KVSVEDFLTD LNNFRTTFMQ AIKENIKKRE 1020
AEEKEKRVRI AKELAERERL ERQQKKKRLL EMKTEGDETG VMDNLLEALQ SGAAFRDRRK 1080
RTPMPKDVRQ SLSPMSQRPV LKVCNHENQK VQLTEGSRSH YNINCNSTRT PVAKELNYNL 1140
DTHTSTGRIK AAEKKEACNV ESNRKKETEL LGSFSKNESV PEVEALLARL RAL 1193 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0030036; P:actin cytoskeleton organization; IEA:InterPro. 
Interpro
 IPR003104; Actin-bd_FH2/DRF_autoreg.
 IPR016024; ARM-type_fold.
 IPR014767; Diaphanous_autoregulatory.
 IPR010465; Drf_DAD.
 IPR010472; Drf_FH3.
 IPR010473; Drf_GTPase-bd.
 IPR015425; FH2_actin-bd.
 IPR014768; GTPase-bd/formin_homology_3. 
Pfam
 PF06345; Drf_DAD
 PF06367; Drf_FH3
 PF06371; Drf_GBD
 PF02181; FH2 
SMART
 SM00498; FH2 
PROSITE
 PS51231; DAD
 PS51444; FH2
 PS51232; GBD_FH3 
PRINTS