UniProt Accession

 5NT3A_MOUSE; Q9D020; Q8CI05; Q9D0D9 

Genbank Protein ID

 AK011525; AK011894; BC038029 

Genbank Nucleotide ID

 BAB27677.2; BAB27901.2; AAH38029.1 

Protein Name

 Cytosolic 5'-nucleotidase 3A 

Protein Synonyms/Alias

 Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase III; cN-III; Lupin; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I 

Gene Name

 Nt5c3a 

Gene Synonyms/Alias

 Nt5c3 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
123	RRKLLQLKEQYYAIE	phosphoglycerylation	[1]
123	RRKLLQLKEQYYAIE	ubiquitination	[2]
166	GIPKAKLKEIVADSD	ubiquitination	[2]

Reference

 [1] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Can act both as nucleotidase and as phosphotransferase (By similarity). 

Sequence Annotation

 REGION 198 200 Substrate binding.
 ACT_SITE 83 83 Nucleophile.
 ACT_SITE 85 85 Proton donor.
 METAL 83 83 Magnesium.
 METAL 85 85 Magnesium; via carbonyl oxygen.
 METAL 272 272 Magnesium.
 BINDING 247 247 Substrate.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 331 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0008253; F:5'-nucleotidase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR023214; HAD-like_dom.
 IPR006383; HAD-SF_hydro_IB_PSP-like.
 IPR006434; Pyrimidine_nucleotidase_eu. 

Pfam

 PF05822; UMPH-1 

SMART

  

PROSITE

  

PRINTS