CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011550
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase UBR2 
Protein Synonyms/Alias
 Ubiquitin-protein ligase E3 component N-recognin-1 homolog 
Gene Name
 UBR2 
Gene Synonyms/Alias
 YLR024C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
709ESLTNSRKAISPDNAubiquitination[1, 2, 3]
1291LFSGKQTKDSAIQENubiquitination[4]
Reference
 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 E3 ubiquitin-protein ligase which probably functions outside the N-end rule pathway, since it lacks the residues essential for the degradation of N-end rule substrates. Mediates RPN4 ubiquitination and subsequent degradation. 
Sequence Annotation
 ZN_FING 96 172 UBR-type.
 ZN_FING 1241 1362 RING-type; atypical.
 REGION 1134 1240 Interaction with UBC2.
 MOD_RES 712 712 Phosphoserine.
 CROSSLNK 709 709 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1872 AA 
Protein Sequence
MEDSDLSITN IRDFLTELPK LAKCEYSETT SYLLWKTLNL RLKHSDNDIN WRSLVSILNS 60
EAWENEKYRD ILNGRKWRTL EFENDHHSVG NMHIGTACTR LCFPSETIYY CFTCSTNPLY 120
EICELCFDKE KHVNHSYVAK VVMRPEGRIC HCGDPFAFND PSDAFKCKNE LNNIPISNDN 180
SNVTDDENVI SLLNYVLDFL IDVTVSYKEE AEAHSSERKA SSLMHPNQNS ITDDIMEKHE 240
CEPLVNDENF VFFDNNWSNT RKEAHMEWAI QIEEEECNVH YMDLASTITR ILNTPVEYAI 300
SITKALEDSH DVVTVLQSEN FFEIDQIAKE FQKENIVVHV RKADDIFKRK LTDDLTDWLY 360
SLCFKAATSL QNKYALRISM LDVWYSHFSK MRVSPTNTNP DFSKINLLGG FLISNEDSDE 420
SWFKPWSLEN IEDERISKIL TNYNERLIRA HSPNTVSHFY NFYGSRFQYI IINSINILSK 480
KSKFKMLKIM ASLFSLRDES RKFLAAQYID VYLSVLYDAV ASDAKECQVT LMSILGQYTF 540
QDPSIANMTI SSGFIERTIR FAFTLMAFNP EDLMSYLPIS LYNGFKLPTE TIRNRRTIIC 600
FKDLCTIMSA NTVPEELLSN EAIFNAIIES FSEFSNVLPL KRETKEHVEV ENFDFSAFYF 660
FFSSILIMTD GYTRSISLVK DAAFRKQIVL KLLDVAQTRE FESLTNSRKA ISPDNASTNE 720
NDSNKATLST VRETICNYVA ETINFQVGVN TQYFFNPMSY LFKFVIQWSQ CGRYEPIPAS 780
LTNYINLFEV FQDKQKALYI SESALSTLVL IGQINVGFWV RNGTPITHQA RMYTKYSMRE 840
FTYISDIFNV QFSMAMCNPD ELMVTYLSRW GLKHWANGVP MYDYPDTETT VAVVNECILL 900
LIQLLTEVRS LVMKSSKEGF ERTFKSEIIH ALCFDTCSYA QIVNCIPEHI TKHPSFDIYL 960
EKYANYTSPV SLTDNGIFVL KEKYKDEIDP YYIGLSSSRR YDVEKNIRLN MANLKKMKYE 1020
DTFVPAKKVK DLLKNTLFSG LYSISSVNTF GLFLKNTLDH IIKYDYDNLL PRVVHLIHLC 1080
VVNNLNEFMG ILWHEYAIVD TEFCHYHSIG SILYYCLLKD NFSESHGKIR EIFRYLMETA 1140
PHVNVNSYLR EQTTSYTPGI LWPTKEDKSH KDKEFERKKH LARLRKKKLM KKLAQQQMKF 1200
MENNSVDTSD ISTPRTTSPS LSPTRINAEN SSNTINSCCD DDCVFCKMPK DDDVFVYFSY 1260
QERNICDHGI DFTNPTDVNR INSLFSGKQT KDSAIQENPQ DDDGTRLKFT SCEPVLRACG 1320
HGSHTKCLSG HMKSIRGIQN QTTKNIPLSY GSGLIYCPVC NSLSNSFLPK TNDIDKRTSS 1380
QFFMCIEKRS EAEENLDPMS SICIKAAMIL GDLQGKKVTT IEDAYKVVNS VFINTISNTE 1440
LRLRSHKKEG KIVNMERISS QCILTLHLVC ELKSFIYKKF VNSKTFSSEI SRKIWNWNEF 1500
LIKGNNVNLL LYMSQNFDNI DGGKTPQPPN LCIYEMFKRR FHQLLLLLAR DMMRVNFYKD 1560
CRNKIKISSN GSEEPSTSFS YLFNTFKKYV DLFKPDDVRF DFTSLEKIKD FICSLLLESL 1620
SIFCRRTFLL FNIQYDDDGD GDNNNNRSNN FMDVKQREIE LIFRYFKLPN LTHFLKDFFY 1680
NELTQNIERY NDGNDNLRIQ QVIYDMVQNI NTRAYPSPEH IQLIELPLNL SKFSLDNDEI 1740
SNKCDKYEIA VCLLCGQKCH IQKSIALQGY LQGECTDHMR NGCEITSAYG VFLMTGTNAI 1800
YLSYGKRGTF YAAPYLSKYG ETNEDYKFGT PVYLNRARYA NLANEIVFGN MIPHIVFRLT 1860
DGSADLGGWE TM 1872 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006513; P:protein monoubiquitination; IDA:SGD. 
Interpro
 IPR003126; Znf_N-recognin.
 IPR013993; Znf_N-recognin_met.
 IPR001841; Znf_RING. 
Pfam
 PF02207; zf-UBR 
SMART
 SM00184; RING
 SM00396; ZnF_UBR1 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2
 PS51157; ZF_UBR 
PRINTS