CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010941
UniProt Accession
Genbank Protein ID
 M92905 
Genbank Nucleotide ID
Protein Name
 Voltage-dependent N-type calcium channel subunit alpha-1B 
Protein Synonyms/Alias
 Brain calcium channel III; BIII; Calcium channel, L type, alpha-1 polypeptide isoform 5; Voltage-gated calcium channel subunit alpha Cav2.2 
Gene Name
 Cacna1b 
Gene Synonyms/Alias
 Cach5; Cacnl1a5 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
1107EGQAEGKKEAEADDVubiquitination[1]
1823QCDAELRKEISSVWAubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin- IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons. 
Sequence Annotation
 REPEAT 82 359 I.
 REPEAT 469 713 II.
 REPEAT 1135 1421 III.
 REPEAT 1458 1711 IV.
 DOMAIN 1724 1759 EF-hand.
 NP_BIND 452 459 ATP (Potential).
 REGION 379 396 Binding to the beta subunit.
 MOD_RES 746 746 Phosphoserine (By similarity).
 MOD_RES 784 784 Phosphoserine (By similarity).
 MOD_RES 1719 1719 Phosphoserine; by PKA (Potential).
 MOD_RES 2253 2253 Phosphoserine (By similarity).
 CARBOHYD 256 256 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1563 1563 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1675 1675 N-linked (GlcNAc...) (Potential).  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Calcium; Calcium channel; Calcium transport; Complete proteome; Disulfide bond; Glycoprotein; Ion channel; Ion transport; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2336 AA 
Protein Sequence
MVRFGDELGG RYGGTGGGER ARGGGAGGAG GPGQGGLPPG QRVLYKQSIA QRARTMALYN 60
PIPVKQNCFT VNRSLFVFSE DNVVRKYAKR ITEWPPFEYM ILATIIANCI VLALEQHLPD 120
GDKTPMSERL DDTEPYFIGI FCFEAGIKII ALGFVFHKGS YLRNGWNVMD FVVVLTEILA 180
TAGTDFDLRT LRAVRVLRPL KLVSGIPSLQ VVLKSIMKAM VPLLQIGLLL FFAILMFAII 240
GLEFYMGKFH KACFPNSTDA EPVGDFPCGK EAPARLCDSD TECREYWPGP NFGITNFDNI 300
LFAILTVFQC ITMEGWTDIL YNTNDAAGNT WNWLYFIPLI IIGSFFMLNL VLGVLSGEFA 360
KERERVENRR AFLKLRRQQQ IERELNGYLE WIFKAEEVML AEEDKNAEEK SPLDAVLKRA 420
ATKKSRNDLI HAEEGEDRFV DLCAAGSPFA RASLKSGKTE SSSYFRRKEK MFRFLIRRMV 480
KAQSFYWVVL CVVALNTLCV AMVHYNQPQR LTTALYFAEF VFLGLFLTEM SLKMYGLGPR 540
SYFRSSFNCF DFGVIVGSIF EVVWAAIKPG TSFGISVLRA LRLLRIFKVT KYWNSLRNLV 600
VSLLNSMKSI ISLLFLLFLF IVVFALLGMQ LFGGQFNFQD ETPTTNFDTF PAAILTVFQI 660
LTGEDWNAVM YHGIESQGGV SKGMFSSFYF IVLTLFGNYT LLNVFLAIAV DNLANAQELT 720
KDEEEMEEAA NQKLALQKAK EVAEVSPMSA ANISIAARQQ NSAKARSVWE QRASQLRLQN 780
LRASCEALYS EMDPEERLRY ASTRHVRPDM KTHMDRPLVV EPGRDGLRGP AGNKSKPEGT 840
EATEGADPPR RHHRHRDRDK TSASTPAGGE QDRTDCPKAE STETGAREER ARPRRSHSKE 900
APGADTQVRC ERSRRHHRRG SPEEATEREP RRHRAHRHAQ DSSKEGKEGT APVLVPKGER 960
RARHRGPRTG PRETENSEEP TRRHRAKHKV PPTLEPPERE VAEKESNVVE GDKETRNHQP 1020
KEPRCDLEAI AVTGVGSLHM LPSTCLQKVD EQPEDADNQR NVTRMGSQPS DPSTTVHVPV 1080
TLTGPPGEAT VVPSANTDLE GQAEGKKEAE ADDVLRRGPR PIVPYSSMFC LSPTNLLRRF 1140
CHYIVTMRYF EMVILVVIAL SSIALAAEDP VRTDSFRNNA LKYMDYIFTG VFTFEMVIKM 1200
IDLGLLLHPG AYFRDLWNIL DFIVVSGALV AFAFSSFMGG SKGKDINTIK SLRVLRVLRP 1260
LKTIKRLPKL KAVFDCVVNS LKNVLNILIV YMLFMFIFAV IAVQLFKGKF FYCTDESKEL 1320
ERDCRGQYLD YEKEEVEAQP RQWKKYDFHY DNVLWALLTL FTVSTGEGWP MVLKHSVDAT 1380
YEEQGPSPGF RMELSIFYVV YFVVFPFFFV NIFVALIIIT FQEQGDKVMS ECSLEKNERA 1440
CIDFAISAKP LTRYMPQNKQ SFQYKTWTFV VSPPFEYFIM AMIALNTVVL MMKFYDAPYE 1500
YELMLKCLNI VFTSMFSLEC ILKIIAFGVL NYFRDAWNVF DFVTVLGSIT DILVTEIANN 1560
FINLSFLRLF RAARLIKLCR QGYTIRILLW TFVQSFKALP YVCLLIAMLF FIYAIIGMQV 1620
FGNIALDDGT SINRHNNFRT FLQALMLLFR SATGEAWHEI MLSCLGNRAC DPHANASECG 1680
SDFAYFYFVS FIFLCSFLML NLFVAVIMDN FEYLTRDSSI LGPHHLDEFI RVWAEYDPAA 1740
CGRISYNDMF EMLKHMSPPL GLGKKCPARV AYKRLVRMNM PISNEDMTVH FTSTLMALIR 1800
TALEIKLAPA GTKQHQCDAE LRKEISSVWA NLPQKTLDLL VPPHKPDEMT VGKVYAALMI 1860
FDFYKQNKTT RDQTHQAPGG LSQMGPVSLF HPLKATLEQT QPAVLRGARV FLRQKSATSL 1920
SNGGAIQTQE SGIKESLSWG TQRTQDVLYE ARAPLERGHS AEIPVGQPGA LAVDVQMQNM 1980
TLRGPDGEPQ PGLESQGRAA SMPRLAAETQ PAPNASPMKR SISTLAPRPH GTQLCNTVLD 2040
RPPPSQVSHH HHHRCHRRRD KKQRSLEKGP SLSVDTEGAP STAAGSGLPH GEGSTGCRRE 2100
RKQERGRSQE RRQPSSSSSE KQRFYSCDRF GSREPPQPKP SLSSHPISPT AALEPGPHPQ 2160
GSGSVNGSPL MSTSGASTPG RGGRRQLPQT PLTPRPSITY KTANSSPVHF AEGQSGLPAF 2220
SPGRLSRGLS EHNALLQKEP LSQPLASGSR IGSDPYLGQR LDSEASAHNL PEDTLTFEEA 2280
VATNSGRSSR TSYVSSLTSQ SHPLRRVPNG YHCTLGLSTG VRARHSYHHP DQDHWC 2336 
Gene Ontology
 GO:0043679; C:axon terminus; IDA:RGD.
 GO:0043198; C:dendritic shaft; IDA:RGD.
 GO:0043025; C:neuronal cell body; IDA:RGD.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0005891; C:voltage-gated calcium channel complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008331; F:high voltage-gated calcium channel activity; IDA:RGD.
 GO:0070509; P:calcium ion import; IMP:RGD.
 GO:0001956; P:positive regulation of neurotransmitter secretion; IMP:RGD.
 GO:0045471; P:response to ethanol; IEP:RGD.
 GO:0033574; P:response to testosterone stimulus; IMP:RGD.
 GO:0019233; P:sensory perception of pain; IMP:RGD. 
Interpro
 IPR002048; EF_hand_dom.
 IPR005821; Ion_trans_dom.
 IPR027359; K_channel_four-helix_dom.
 IPR014873; VDCC_a1su_IQ.
 IPR005447; VDCC_N_a1su.
 IPR002077; VDCCAlpha1. 
Pfam
 PF08763; Ca_chan_IQ
 PF00520; Ion_trans 
SMART
 SM01062; Ca_chan_IQ 
PROSITE
 PS50222; EF_HAND_2 
PRINTS
 PR00167; CACHANNEL.
 PR01631; NVDCCALPHA1.