CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012125
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sequestosome-1 
Protein Synonyms/Alias
 EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62 
Gene Name
 SQSTM1 
Gene Synonyms/Alias
 ORCA; OSIL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
81GLHRGHTKLAFPSPFubiquitination[1]
105SRWLRKVKHGHFGWPubiquitination[1]
180IDVEHGGKRSRLTPVubiquitination[1]
211SCCSDPSKPGGNVEGubiquitination[1]
294QEGPTGLKEAALYPHubiquitination[1]
336LTRLLQTKNYDIGAAubiquitination[1]
351LDTIQYSKHPPPL**ubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. 
Sequence Annotation
 DOMAIN 20 102 OPR.
 DOMAIN 389 434 UBA.
 ZN_FING 122 167 ZZ-type.
 REGION 1 50 Interaction with LCK.
 REGION 43 107 Interaction with PRKCZ and dimerization
 REGION 50 80 Interaction with PAWR.
 REGION 122 224 Interaction with GABRR3 (By similarity).
 REGION 170 220 LIM protein-binding (LB).
 REGION 269 440 Interaction with NTRK1 (By similarity).
 REGION 321 342 MAP1LC3B-binding.
 MOTIF 228 233 TRAF6-binding.
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 148 148 Phosphotyrosine.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 207 207 Phosphoserine.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 266 266 Phosphoserine.
 MOD_RES 269 269 Phosphothreonine.
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 328 328 Phosphoserine.
 MOD_RES 332 332 Phosphoserine.
 MOD_RES 355 355 Phosphoserine.
 MOD_RES 361 361 Phosphoserine.
 MOD_RES 365 365 Phosphoserine (By similarity).
 MOD_RES 366 366 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Autophagy; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Differentiation; Direct protein sequencing; Disease mutation; Endoplasmic reticulum; Endosome; Immunity; Lysosome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 440 AA 
Protein Sequence
MAMSYVKDDI FRIYIKEKKE CRRDHRPPCA QEAPRNMVHP NVICDGCNGP VVGTRYKCSV 60
CPDYDLCSVC EGKGLHRGHT KLAFPSPFGH LSEGFSHSRW LRKVKHGHFG WPGWEMGPPG 120
NWSPRPPRAG EARPGPTAES ASGPSEDPSV NFLKNVGESV AAALSPLGIE VDIDVEHGGK 180
RSRLTPVSPE SSSTEEKSSS QPSSCCSDPS KPGGNVEGAT QSLAEQMRKI ALESEGRPEE 240
QMESDNCSGG DDDWTHLSSK EVDPSTGELQ SLQMPESEGP SSLDPSQEGP TGLKEAALYP 300
HLPPEADPRL IESLSQMLSM GFSDEGGWLT RLLQTKNYDI GAALDTIQYS KHPPPL 356 
Gene Ontology
 GO:0005776; C:autophagic vacuole; IDA:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:UniProtKB.
 GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000407; C:pre-autophagosomal structure; IEA:Compara.
 GO:0019901; F:protein kinase binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; NAS:UniProtKB.
 GO:0030971; F:receptor tyrosine kinase binding; TAS:ProtInc.
 GO:0042169; F:SH2 domain binding; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016197; P:endosomal transport; TAS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; TAS:UniProtKB.
 GO:0016236; P:macroautophagy; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; TAS:UniProtKB.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0008104; P:protein localization; TAS:UniProtKB.
 GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0046578; P:regulation of Ras protein signal transduction; NAS:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc. 
Interpro
 IPR000270; OPR_PB1.
 IPR009060; UBA-like.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR000433; Znf_ZZ. 
Pfam
 PF00564; PB1
 PF00569; ZZ 
SMART
 SM00666; PB1
 SM00165; UBA
 SM00291; ZnF_ZZ 
PROSITE
 PS50030; UBA
 PS01357; ZF_ZZ_1
 PS50135; ZF_ZZ_2 
PRINTS