CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007628
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neutral amino acid transporter A 
Protein Synonyms/Alias
 Alanine/serine/cysteine/threonine transporter 1; ASCT-1; SATT; Solute carrier family 1 member 4 
Gene Name
 SLC1A4 
Gene Synonyms/Alias
 ASCT1; SATT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
483HLNQKATKKGEQELAubiquitination[1]
484LNQKATKKGEQELAEubiquitination[1, 2, 3]
493EQELAEVKVEAIPNCubiquitination[1, 2, 3, 4, 5]
501VEAIPNCKSEEETSPubiquitination[1, 2, 4, 5, 6]
528SAPELESKESVL***ubiquitination[2, 4, 5, 6, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence. 
Sequence Annotation
 MOD_RES 506 506 Phosphothreonine (By similarity).
 MOD_RES 507 507 Phosphoserine.
 CARBOHYD 201 201 N-linked (GlcNAc...).
 CARBOHYD 206 206 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 532 AA 
Protein Sequence
MEKSNETNGY LDSAQAGPAA GPGAPGTAAG RARRCAGFLR RQALVLLTVS GVLAGAGLGA 60
ALRGLSLSRT QVTYLAFPGE MLLRMLRMII LPLVVCSLVS GAASLDASCL GRLGGIAVAY 120
FGLTTLSASA LAVALAFIIK PGSGAQTLQS SDLGLEDSGP PPVPKETVDS FLDLARNLFP 180
SNLVVAAFRT YATDYKVVTQ NSSSGNVTHE KIPIGTEIEG MNILGLVLFA LVLGVALKKL 240
GSEGEDLIRF FNSLNEATMV LVSWIMWYVP VGIMFLVGSK IVEMKDIIVL VTSLGKYIFA 300
SILGHVIHGG IVLPLIYFVF TRKNPFRFLL GLLAPFATAF ATCSSSATLP SMMKCIEENN 360
GVDKRISRFI LPIGATVNMD GAAIFQCVAA VFIAQLNNVE LNAGQIFTIL VTATASSVGA 420
AGVPAGGVLT IAIILEAIGL PTHDLPLILA VDWIVDRTTT VVNVEGDALG AGILHHLNQK 480
ATKKGEQELA EVKVEAIPNC KSEEETSPLV THQNPAGPVA SAPELESKES VL 532 
Gene Ontology
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005887; C:integral to plasma membrane; IC:UniProtKB.
 GO:0005882; C:intermediate filament; ISS:UniProtKB.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005254; F:chloride channel activity; IDA:UniProtKB.
 GO:0015180; F:L-alanine transmembrane transporter activity; IDA:UniProtKB.
 GO:0015184; F:L-cystine transmembrane transporter activity; IDA:UniProtKB.
 GO:0015186; F:L-glutamine transmembrane transporter activity; TAS:BHF-UCL.
 GO:0034590; F:L-hydroxyproline transmembrane transporter activity; IDA:UniProtKB.
 GO:0015193; F:L-proline transmembrane transporter activity; IDA:UniProtKB.
 GO:0015194; F:L-serine transmembrane transporter activity; IDA:UniProtKB.
 GO:0015195; F:L-threonine transmembrane transporter activity; IDA:UniProtKB.
 GO:0017153; F:sodium:dicarboxylate symporter activity; IEA:InterPro.
 GO:0050890; P:cognition; IMP:UniProtKB.
 GO:0035249; P:synaptic transmission, glutamatergic; NAS:UniProtKB. 
Interpro
 IPR001991; Na-dicarboxylate_symporter.
 IPR018107; Na-dicarboxylate_symporter_CS. 
Pfam
 PF00375; SDF 
SMART
  
PROSITE
 PS00713; NA_DICARBOXYL_SYMP_1
 PS00714; NA_DICARBOXYL_SYMP_2 
PRINTS
 PR00173; EDTRNSPORT.