UniProt Accession

 RPAB5_YEAST; P22139; D6W2R6 

Genbank Protein ID

 M60479; S62098; L11274; Z75118; AY558433; BK006948 

Genbank Nucleotide ID

 AAA34995.1; AAB27020.1; AAB59318.1; CAA99425.1; AAS56759.1; DAA10982.1 

Protein Name

 DNA-directed RNA polymerases I, II, and III subunit RPABC5 

Protein Synonyms/Alias

 RNA polymerases I, II, and III subunit ABC5; ABC10-beta; ABC8; DNA-directed RNA polymerases I, II, and III 8.3 kDa polypeptide 

Gene Name

 RPB10 

Gene Synonyms/Alias

 YOR210W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
17	CGKVVGDKWESYLNL	ubiquitination	[1]
59	THVDLIEKFLRYNPL	ubiquitination	[1, 2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RBP10 is part of the core element with the central large cleft. 

Sequence Annotation

 METAL 7 7 Zinc (By similarity).
 METAL 10 10 Zinc (By similarity).
 METAL 44 44 Zinc (By similarity).
 METAL 45 45 Zinc (By similarity).  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA-directed RNA polymerase; Metal-binding; Nucleus; Reference proteome; Transcription; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 70 AA 

Protein Sequence

Gene Ontology

 GO:0005736; C:DNA-directed RNA polymerase I complex; IDA:SGD.
 GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:SGD.
 GO:0005666; C:DNA-directed RNA polymerase III complex; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IDA:SGD.
 GO:0006360; P:transcription from RNA polymerase I promoter; IDA:SGD.
 GO:0006366; P:transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0042797; P:tRNA transcription from RNA polymerase III promoter; IDA:SGD. 

Interpro

 IPR009057; Homeodomain-like.
 IPR023580; RNA_pol_su_RPB10.
 IPR020789; RNA_pol_suN_Zn-BS.
 IPR000268; RNAP_N/Rpb10. 

Pfam

 PF01194; RNA_pol_N 

SMART

  

PROSITE

 PS01112; RNA_POL_N_8KD 

PRINTS