UniProt Accession

 HSLV_ECOLI; P0A7B8; P31059; P97542; Q2M8M8 

Genbank Protein ID

 L19201; U00096; AP009048; D89965; L14281 

Genbank Nucleotide ID

 AAB03064.1; AAC76914.1; BAE77378.1; BAA14040.1 

Protein Name

 ATP-dependent protease subunit HslV 

Protein Synonyms/Alias

 Heat shock protein HslV 

Gene Name

 hslV 

Gene Synonyms/Alias

 htpO; yiiC; b3932; JW3903 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
64	LFELFERKLEMHQGH	acetylation	[1]
74	MHQGHLVKAAVELAK	acetylation	[1, 2]
81	KAAVELAKDWRTDRM	acetylation	[1, 2]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery. The complex has been shown to be involved in the specific degradation of heat shock induced transcription factors such as RpoH and SulA. In addition, small hydrophobic peptides are also hydrolyzed by HslV. HslV has weak protease activity even in the absence of HslU, but this activity is induced more than 100-fold in the presence of HslU. HslU recognizes protein substrates and unfolds these before guiding them to HslV for hydrolysis. HslV is not believed to degrade folded proteins. 

Sequence Annotation

 ACT_SITE 2 2
 METAL 157 157 Sodium; via carbonyl oxygen.
 METAL 160 160 Sodium; via carbonyl oxygen.
 METAL 163 163 Sodium; via carbonyl oxygen.  

Keyword

 3D-structure; Allosteric enzyme; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Metal-binding; Protease; Reference proteome; Sodium; Stress response; Threonine protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 176 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0009376; C:HslUV protease complex; IEA:HAMAP.
 GO:0005839; C:proteasome core complex; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0051603; P:proteolysis involved in cellular protein catabolic process; IEA:InterPro.
 GO:0009408; P:response to heat; IEP:EcoliWiki. 

Interpro

 IPR022281; ATP-dep_Prtase_HsIV_su.
 IPR001353; Proteasome_sua/b. 

Pfam

 PF00227; Proteasome 

SMART

  

PROSITE

  

PRINTS